Reversible Energy-Transfer Switching on a DNA Scaffold

• We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNAscaffold can be reversibly controlled by photochromicswitching of a spiropyran derivative. With the spiropyranin the closed spiro isomeric form, FRET occurs freelybetween PB and A488. UV-induced isomerization to theopen merocyanine form shuts down the FRET process byefficient quenching of the PB excited state. The process isreversed by exposure to visible light, triggering theisomerization to the spiro isomer.

Subject headings

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

Publication and Content Type

art (subject category)

ref (subject category)