Continuous Cultivation of Dilute-Acid Hydrolysates to Ethanol by Immobilized Saccharomyces cerevisiae

Taherzadeh Esfahani, Mohammad,1965Chalmers tekniska högskola,Chalmers University of Technology (author)

The continuous cultivation of immobilized Saccharomyces cerevisiae CBS 8066 on dilute-acid hydrolysates of forest residuals was investigated. The yeast cells were immobilized in 2–4% Ca-alginate beads. The 2% beads were not stable. However, the 3 and 4% beads were stable for at least 3 wk when an extra resource of calcium ions was available in the medium. The continuous cultivation of a dilute-acid hydrolysate by the immobilized cells at dilution rates of 0.3, 0.5, and 0.6 h−1 resulted in 86, 83, and 79% sugar consumption, respectively, and an ethanol yield between 0.45 and 0.48 g/g. The hydrolysate was fermentable at a dilution rate of 0.1 h−1 in a free-cell system but washed out at a dilution rate of 0.2 h−1. The continuous cultivation of a more inhibiting hydrolysate was not successful by either free- or immobilized-cell systems even at a low dilution rate of 0.07 h−1. However, when the hydrolysate was overlimed, it was fermentable by the immobilized cells at a dilution rate of 0.2 h−1.

Niklasson, Claes,1957Chalmers tekniska högskola,Chalmers University of Technology,Dept. of Chemical Reaction Eng., Chalmers University of Technology(Swepub:cth)claesn (author)
Millati, Ria,1972Chalmers tekniska högskola,Chalmers University of Technology,Dept. of Chemical Reaction Eng., Chalmers University of Technology(Swepub:cth)millati (author)
Taherzadeh, Mohammad J,1965-Dept. of Chemical Reaction Eng., Chalmers University of Technology(Swepub:hb)MJT (author)
Chalmers tekniska högskolaDept. of Chemical Reaction Eng., Chalmers University of Technology (creator_code:org_t)

By the author/editor: Taherzadeh Esfah ...; Niklasson, Claes ...; Millati, Ria, 19 ...; Taherzadeh, Moha ...

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ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Industrial Biote ...; and Bioprocess Techn ...

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