Genomic mining of Geobacillus stearothermophilus GF16 for xylose production from hemicellulose-rich biomasses using secreted enzymes

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Carbonaro, MiriamUniversita Degli Studi Di Napoli Federico Ii,University of Naples Federico II (author)

Genomic mining of Geobacillus stearothermophilus GF16 for xylose production from hemicellulose-rich biomasses using secreted enzymes

Article/chapterEnglish2024

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2024
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LIBRIS-ID:oai:research.chalmers.se:3c2f3b64-847c-451d-8652-f80da363d5b6
https://doi.org/10.1016/j.nbt.2024.04.002DOI
https://research.chalmers.se/publication/541556URI

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Language:English
Summary in:English

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The valorization of lignocellulosic biomass, derived from various bio-waste materials, has received considerable attention as a sustainable approach to improve production chains while reducing environmental impact. Microbial enzymes have emerged as key players in the degradation of polysaccharides, offering versatile applications in biotechnology and industry. Among these enzymes, glycoside hydrolases (GHs) play a central role. Xylanases, in particular, are used in a wide range of applications and are essential for the production of xylose, which can be fermented into bioethanol or find use in many other industries. Currently, fungal secretomes dominate as the main reservoir of lignocellulolytic enzymes, but thermophilic microorganisms offer notable advantages in terms of enzyme stability and production efficiency. Here we present the genomic characterization of Geobacillus stearothermophilus GF16 to identify genes encoding putative enzymes involved in lignocellulose degradation. Thermostable GHs secreted by G. stearothermophilus GF16 were investigated and found to be active on different natural polysaccharides and synthetic substrates, revealing an array of inducible GH activities. In particular, the concentrated secretome possesses significant thermostable xylanase and β-xylosidase activities (5 ×103 U/L and 1.7 ×105 U/L, respectively), highlighting its potential for application in biomass valorization. We assessed the hemicellulose hydrolysis capabilities of various agri-food wastes using the concentrated secretome of the strain cultivated on xylan. An impressive 300-fold increase in xylose release compared to a commercially available cocktail was obtained with the secretome, underscoring the remarkable efficacy of this approach.

Subject headings and genre

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
TEKNIK OCH TEKNOLOGIER Industriell bioteknik Bioenergi hsv//swe
ENGINEERING AND TECHNOLOGY Industrial Biotechnology Bioenergy hsv//eng
NATURVETENSKAP Biologi Mikrobiologi hsv//swe
NATURAL SCIENCES Biological Sciences Microbiology hsv//eng
TEKNIK OCH TEKNOLOGIER Industriell bioteknik Biokatalys och enzymteknik hsv//swe
ENGINEERING AND TECHNOLOGY Industrial Biotechnology Biocatalysis and Enzyme Technology hsv//eng
Geobacillus stearothermophilus GF16
Xylan
Carbohydrate active enzymes
Agri-food waste valorization
Bacterial secretome

Added entries (persons, corporate bodies, meetings, titles ...)

Aulitto, Martina,1991Universita Degli Studi Di Napoli Federico Ii,University of Naples Federico II(Swepub:cth)aulitto (author)
Mazurkewich, Scott,1982Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)scottma (author)
Fraia, Alessia DiUniversita Degli Studi Di Napoli Federico Ii,University of Naples Federico II (author)
Contursi, PatriziaUniversita Degli Studi Di Napoli Federico Ii,University of Naples Federico II (author)
Limauro, DanilaUniversita Degli Studi Di Napoli Federico Ii,University of Naples Federico II (author)
Larsbrink, Johan,1982Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)jolarsb (author)
Fiorentino, GabriellaUniversita Degli Studi Di Napoli Federico Ii,University of Naples Federico II (author)
Universita Degli Studi Di Napoli Federico IiChalmers tekniska högskola (creator_code:org_t)

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In:New Biotechnology82, s. 14-241876-43471871-6784

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NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Industrial Biote ...; and Bioenergy

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