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Effects of x-ray fr...
Effects of x-ray free-electron laser pulse intensity on the Mn K beta(1,3) x-ray emission spectrum in photosystem II-A case study for metalloprotein crystals and solutions
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- Fransson, Thomas (författare)
- KTH,Teoretisk kemi och biologi,KTH Royal Inst Technol, Dept Theoret Chem & Biol, Stockholm, Sweden.
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- Alonso-Mori, Roberto (författare)
- SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA.
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- Chatterjee, Ruchira (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Cheah, Mun Hon (författare)
- Uppsala universitet,Molekylär biomimetik
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- Ibrahim, Mohamed (författare)
- Humboldt Univ, Dept Biol, D-10099 Berlin, Germany.
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- Hussein, Rana (författare)
- Humboldt Univ, Dept Biol, D-10099 Berlin, Germany.
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- Zhang, Miao (författare)
- Humboldt Univ, Dept Biol, D-10099 Berlin, Germany.
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- Fuller, Franklin (författare)
- SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA.
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- Gul, Sheraz (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Kim, In-Sik (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Simon, Philipp S. (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.,SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA.
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- Bogacz, Isabel (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Makita, Hiroki (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- de Lichtenberg, Casper (författare)
- Uppsala universitet,Molekylär biomimetik,Umeå Univ, Dept Chem, SE-90187 Umeå, Sweden.;Univ Copenhagen, Sect Biomol Sci, Dept Biol, DK-2200 Copenhagen N, Denmark.
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- Song, Sanghoon (författare)
- SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA.
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- Batyuk, Alexander (författare)
- SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA.
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- Sokaras, Dimosthenis (författare)
- SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, Menlo Pk, CA 94025 USA.
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- Massad, Ramzi (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Doyle, Margaret (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Britz, Alexander (författare)
- SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA.;SLAC Natl Accelerator Lab, Stanford PULSE Inst, Menlo Pk, CA 94025 USA.
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- Weninger, Clemens (författare)
- Lund Univ, MAX Lab 4, Lund, Sweden.
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- Zouni, Athina (författare)
- Humboldt Univ, Dept Biol, D-10099 Berlin, Germany.
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- Messinger, Johannes, 1963- (författare)
- Uppsala universitet,Molekylär biomimetik,Umeå Univ, Dept Chem, SE-90187 Umeå, Sweden.
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- Yachandra, Vittal K. (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Yano, Junko (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Kern, Jan (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Bergmann, Uwe (författare)
- Univ Wisconsin, Dept Phys, Madison, WI 53706 USA.
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(creator_code:org_t)
- AIP Publishing, 2021
- 2021
- Engelska.
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Ingår i: Structural Dynamics. - : AIP Publishing. - 2329-7778. ; 8:6
- Relaterad länk:
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https://doi.org/10.1...
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https://aca.scitatio...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- In the last ten years, x-ray free-electron lasers (XFELs) have been successfully employed to characterize metalloproteins at room temperature using various techniques including x-ray diffraction, scattering, and spectroscopy. The approach has been to outrun the radiation damage by using femtosecond (fs) x-ray pulses. An example of an important and damage sensitive active metal center is the Mn4CaO5 cluster in photosystem II (PS II), the catalytic site of photosynthetic water oxidation. The combination of serial femtosecond x-ray crystallography and K beta x-ray emission spectroscopy (XES) has proven to be a powerful multimodal approach for simultaneously probing the overall protein structure and the electronic state of the Mn4CaO5 cluster throughout the catalytic (Kok) cycle. As the observed spectral changes in the Mn4CaO5 cluster are very subtle, it is critical to consider the potential effects of the intense XFEL pulses on the K beta XES signal. We report here a systematic study of the effects of XFEL peak power, beam focus, and dose on the Mn K beta(1,3) XES spectra in PS II over a wide range of pulse parameters collected over seven different experimental runs using both microcrystal and solution PS II samples. Our findings show that for beam intensities ranging from & SIM;5 x 10(15) to 5 x 10(17) W/cm(2) at a pulse length of & SIM;35 fs, the spectral effects are small compared to those observed between S-states in the Kok cycle. Our results provide a benchmark for other XFEL-based XES studies on metalloproteins, confirming the viability of this approach.& nbsp;
Ämnesord
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
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Fransson, Thomas
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Alonso-Mori, Rob ...
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Chatterjee, Ruch ...
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Cheah, Mun Hon
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Ibrahim, Mohamed
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Hussein, Rana
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Zhang, Miao
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Fuller, Franklin
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Gul, Sheraz
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Kim, In-Sik
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Simon, Philipp S ...
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Bogacz, Isabel
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Makita, Hiroki
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de Lichtenberg, ...
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Song, Sanghoon
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Batyuk, Alexande ...
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Sokaras, Dimosth ...
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Massad, Ramzi
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Doyle, Margaret
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Britz, Alexander
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Weninger, Clemen ...
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Zouni, Athina
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Messinger, Johan ...
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Yachandra, Vitta ...
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Yano, Junko
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Kern, Jan
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Bergmann, Uwe
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Structural Dynam ...
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