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Sökning: WFRF:(Parekh S.) > (2020-2024) > Effect of ambient t...

  • Paul, Alexandra,1988The University of Texas at Austin, USA,Chalmers tekniska högskola,Chalmers University of Technology (författare)

Effect of ambient temperature on respiratory tract cells exposed to SARS-CoV-2 viral mimicking nanospheres - An experimental study

  • Artikel/kapitelEngelska2021

Förlag, utgivningsår, omfång ...

  • American Vacuum Society,2021

Nummerbeteckningar

  • LIBRIS-ID:oai:research.chalmers.se:3f44cfa3-0784-41fa-96ca-a3313254483e
  • https://research.chalmers.se/publication/523417URI
  • https://doi.org/10.1116/6.0000743DOI
  • https://research.chalmers.se/publication/522124URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:art swepub-publicationtype
  • Ämneskategori:ref swepub-contenttype

Anmärkningar

  • The novel coronavirus caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has reached more than 160 countries and has been declared a pandemic. SARS-CoV-2 infects host cells by binding to the angiotensin-converting enzyme 2 (ACE-2) surface receptor via the spike (S) receptor-binding protein (RBD) on the virus envelope. Global data on a similar infectious disease spread by SARS-CoV-1 in 2002 indicated improved stability of the virus at lower temperatures facilitating its high transmission in the community during colder months (December–February). Seasonal viral transmissions are strongly modulated by temperatures, which can impact viral trafficking into host cells; however, an experimental study of temperature-dependent activity of SARS-CoV-2 is still lacking. We mimicked SARS-CoV-2 with polymer beads coated with the SARS-CoV-2 S protein to study the effect of seasonal temperatures on the binding of virus-mimicking nanospheres to lung epithelia. The presence of the S protein RBD on nanosphere surfaces led to binding by Calu-3 airway epithelial cells via the ACE-2 receptor. Calu-3 and control fibroblast cells with S-RBD-coated nanospheres were incubated at 33 and 37 °C to mimic temperature fluctuations in the host respiratory tract, and we found no temperature dependence in contrast to nonspecific binding of bovine serum ablumin-coated nanospheres. Moreover, the ambient temperature changes from 4 to 40 °C had no effect on S-RBD-ACE-2 ligand-receptor binding and minimal effect on the S-RBD protein structure (up to 40 °C), though protein denaturing occurred at 51 °C. Our results suggest that ambient temperatures from 4 to 40 °C have little effect on the SARS-CoV-2-ACE-2 interaction in agreement with the infection data currently reported.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Kumar, SachinThe University of Texas at Austin, USA (författare)
  • Chatterjee, SayantaniMax Planck Gesellschaft zur Förderung der Wissenschaften e.V. (MPG),Max Planck Society for the Advancement of Science (MPG) (författare)
  • Pütz, SabineMax Planck Gesellschaft zur Förderung der Wissenschaften e.V. (MPG),Max Planck Society for the Advancement of Science (MPG) (författare)
  • Nehra, NatashaThe University of Texas at Austin, USA (författare)
  • Wang, Daniel S.The University of Texas at Austin, USA (författare)
  • Nisar, ArsalanThe University of Texas at Austin, USA (författare)
  • Jennings, Christian M.The University of Texas at Austin, USA (författare)
  • Parekh, Sapun H.The University of Texas at Austin, USA,Max Planck Gesellschaft zur Förderung der Wissenschaften e.V. (MPG),Max Planck Society for the Advancement of Science (MPG) (författare)
  • The University of Texas at Austin, USAChalmers tekniska högskola (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Biointerphases: American Vacuum Society16:11559-41061934-8630

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