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  • Ramon-Azcon, J.Tohoku University (author)

Gelatin methacrylate as a promising hydrogel for 3D microscale organization and proliferation of dielectrophoretically patterned cells

  • Article/chapterEnglish2012

Publisher, publication year, extent ...

  • Royal Society of Chemistry (RSC),2012

Numbers

  • LIBRIS-ID:oai:research.chalmers.se:493c23b4-2876-49d6-8895-2365707aaadc
  • https://research.chalmers.se/publication/171900URI
  • https://doi.org/10.1039/c2lc40213kDOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Establishing the 3D microscale organization of cells has numerous practical applications, such as in determining cell fate (e. g., proliferation, migration, differentiation, and apoptosis) and in making functional tissue constructs. One approach to spatially pattern cells is by dielectrophoresis (DEP). DEP has characteristics that are important for cell manipulation, such as high accuracy, speed, scalability, and the ability to handle both adherent and non-adherent cells. However, widespread application of this method is largely restricted because there is a limited number of suitable hydrogels for cell encapsulation. To date, polyethylene glycol-diacrylate (PEG-DA) and agarose have been used extensively for dielectric patterning of cells. In this study, we propose gelatin methacrylate (GelMA) as a promising hydrogel for use in cell dielectropatterning because of its biocompatibility and low viscosity. Compared to PEG hydrogels, GelMA hydrogels showed superior performance when making cell patterns for myoblast (C2C12) and endothelial (HUVEC) cells as well as in maintaining cell viability and growth. We also developed a simple and robust protocol for co-culture of these cells. Combined application of the GelMA hydrogels and the DEP technique is suitable for creating highly complex microscale tissues with important applications in fundamental cell biology and regenerative medicine in a rapid, accurate, and scalable manner.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Ahadian, S.Tohoku University (author)
  • Obregon, R.Tohoku University (author)
  • Camci-Unal, G.Massachusetts Institute of Technology (MIT),Brigham and Women's Hospital (author)
  • Ostrovidov, S.Tohoku University (author)
  • Hosseini, VahidChalmers tekniska högskola,Chalmers University of Technology (author)
  • Kaji, H.Tohoku University (author)
  • Ino, K.Tohoku University (author)
  • Shiku, H.Tohoku University (author)
  • Khademhosseini, A.Harvard University,Kyung Hee University,Tohoku University,Brigham and Women's Hospital,Massachusetts Institute of Technology (MIT) (author)
  • Matsue, T.Tohoku University (author)
  • Tohoku UniversityMassachusetts Institute of Technology (MIT) (creator_code:org_t)

Related titles

  • In:Lab on a Chip - Miniaturisation for Chemistry and Biology: Royal Society of Chemistry (RSC)12:16, s. 2959-29691473-01891473-0197

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