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- Gunnarsson, Anders,1981Chalmers tekniska högskola,Chalmers University of Technology (author)
Kinetic and thermodynamic characterization of single-mismatch discrimination using single-molecule imaging
- Article/chapterEnglish2009
Publisher, publication year, extent ...
- 2009-06-09
- Oxford University Press (OUP),2009
Numbers
- LIBRIS-ID:oai:research.chalmers.se:54ceb1d7-57c6-4526-81e2-f28ef71befbb
- https://research.chalmers.se/publication/115413URI
- https://doi.org/10.1093/nar/gkp487DOI
Supplementary language notes
- Language:English
- Summary in:English
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- A single-molecule detection setup based on total internal reflection fluorescence (TIRF) microscopy has been used to investigate association and dissociation kinetics of unlabeled 30mer DNA strands. Single-molecule sensitivity was accomplished by letting unlabeled DNA target strands mediate the binding of DNA-modified and fluorescently labeled liposomes to a DNA-modified surface. The liposomes, acting as signal enhancer elements, enabled the number of binding events as well as the residence time for high affinity binders (K-d
Subject headings and genre
- NATURVETENSKAP Biologi Biofysik hsv//swe
- NATURAL SCIENCES Biological Sciences Biophysics hsv//eng
- hybridization
- dissociation
- sequence
- density oligonucleotide arrays
- base-pairs
- surface-plasmon resonance
- dna
- nanoparticles
- microarrays
- nucleotide polymorphisms
- nucleic-acids
Added entries (persons, corporate bodies, meetings, titles ...)
- Jönsson, Peter,1981Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)jopeter (author)
- Zhdanov, Vladimir,1952Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)zhdanov (author)
- Höök, Fredrik,1966Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)fredrikh (author)
- Chalmers tekniska högskola (creator_code:org_t)
Related titles
- In:Nucleic Acids Research: Oxford University Press (OUP)37:14, s. e99 (art no)-0305-10481362-4962
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