WFRF:(Liu Lifang)
Sökning: WFRF:(Liu Lifang) > Genome-scale analys...
- Liu, Lifang,1979Chalmers tekniska högskola,Chalmers University of Technology (författare)
Genome-scale analysis of the high-efficient protein secretion system of Aspergillus oryzae
- Artikel/kapitelEngelska2014
Förlag, utgivningsår, omfång ...
- 2014-06-24
- Springer Science and Business Media LLC,2014
- electronicrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:research.chalmers.se:59609b22-b357-4051-b690-afae05fb585e
- https://doi.org/10.1186/1752-0509-8-73DOI
- https://research.chalmers.se/publication/200793URI
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- Språk:engelska
- Sammanfattning på:engelska
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Klassifikation
Anmärkningar
- Background: The koji mold, Aspergillus oryzae is widely used for the production of industrial enzymes due to its particularly high protein secretion capacity and ability to perform post-translational modifications. However, systemic analysis of its secretion system is lacking, generally due to the poorly annotated proteome. Results: Here we defined a functional protein secretory component list of A. oryzae using a previously reported secretory model of S. cerevisiae as scaffold. Additional secretory components were obtained by blast search with the functional components reported in other closely related fungal species such as Aspergillus nidulans and Aspergillus niger. To evaluate the defined component list, we performed transcriptome analysis on three a-amylase over-producing strains with varying levels of secretion capacities. Specifically, secretory components involved in the ER-associated processes (including components involved in the regulation of transport between ER and Golgi) were significantly up-regulated, with many of them never been identified for A. oryzae before. Furthermore, we defined a complete list of the putative A. oryzae secretome and monitored how it was affected by overproducing amylase. Conclusion: In combination with the transcriptome data, the most complete secretory component list and the putative secretome, we improved the systemic understanding of the secretory machinery of A. oryzae in response to high levels of protein secretion. The roles of many newly predicted secretory components were experimentally validated and the enriched component list provides a better platform for driving more mechanistic studies of the protein secretory pathway in this industrially important fungus.
Ämnesord och genrebeteckningar
- NATURVETENSKAP Biologi Bioinformatik och systembiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Bioinformatics and Systems Biology hsv//eng
- ENDOPLASMIC-RETICULUM
- Aspergillus oryzae
- Comparative genomics
- SACCHAROMYCES-CEREVISIAE
- AMYLASE GENES
- Mathematical & Computational Biology
- 1988
- Transcriptome
- CELL-WALLS
- V6
- STRESS-RESPONSE
- alpha-amylase
- GENE-EXPRESSION
- INDUCTION
- Secretory pathway
- P1419
- FILAMENTOUS FUNGI
- NIGER
- BIO-TECHNOLOGY
- RISTENSEN T
- TRANSCRIPTIONAL ACTIVATOR
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Feizi, Amir,1980Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)feizi (författare)
- Österlund, Tobias,1984Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)tobiaso (författare)
- Hjort, C.Novozymes A/S (författare)
- Nielsen, Jens B,1962Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)nielsenj (författare)
- Chalmers tekniska högskolaNovozymes A/S (creator_code:org_t)
Sammanhörande titlar
- Ingår i:BMC Systems Biology: Springer Science and Business Media LLC8:731752-0509
Internetlänk
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