Search: WFRF:(Joyce Paul 1989) >
Independent Size an...
Independent Size and Fluorescence Emission Determination of Individual Biological Nanoparticles Reveals that Lipophilic Dye Incorporation Does Not Scale with Particle Size
-
- Jõemetsa, Silver, 1990 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Joyce, Paul, 1989 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Lubart, Quentin, 1989 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
show more...
-
- Mapar, Mokhtar, 1983 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Celauro, Emanuele, 1980 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Agnarsson, Björn, 1977 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Block, Stephan, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Bally, Marta, 1981 (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Department of Physics, Chalmers University of Technology, Göteborg, Sweden,Chalmers tekniska högskola,Chalmers University of Technology
-
- Esbjörner Winters, Elin, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Jeffries, Gavin, 1980 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Höök, Fredrik, 1966 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
Esbjorner, Elin K. (author)
-
show less...
-
(creator_code:org_t)
- 2020-07-27
- 2020
- English.
-
In: Langmuir. - : American Chemical Society (ACS). - 1520-5827 .- 0743-7463. ; 36:33, s. 9693-9700
- Related links:
-
https://research.cha...
-
show more...
-
https://doi.org/10.1...
-
https://research.cha...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Advancements in nanoparticle characterization techniques are critical for improving the understanding of how biological nanoparticles (BNPs) contribute to different cellular processes, such as cellular communication, viral infection, as well as various drug-delivery applications. Since BNPs are intrinsically heterogeneous, there is a need for characterization methods that are capable of providing information about multiple parameters simultaneously, preferably at the single-nanoparticle level. In this work, fluorescence microscopy was combined with surface-based two-dimensional flow nanometry, allowing for simultaneous and independent determination of size and fluorescence emission of individual BNPs. In this way, the dependence of the fluorescence emission of the commonly used self-inserting lipophilic dye 3,3′-dioctadecyl-5,5′-di(4-sulfophenyl)oxacarbocyanine (SP-DiO) could successfully be correlated with nanoparticle size for different types of BNPs, including synthetic lipid vesicles, lipid vesicles derived from cellular membrane extracts, and extracellular vesicles derived from human SH-SY5Y cell cultures; all vesicles had a radius, r, of ∼50 nm and similar size distributions. The results demonstrate that the dependence of fluorescence emission of SP-DiO on nanoparticle size varies significantly between the different types of BNPs, with the expected dependence on membrane area, r2, being observed for synthetic lipid vesicles, while a significant weaker dependence on size was observed for BNPs with more complex composition. The latter observation is attributed to a size-dependent difference in membrane composition, which may influence either the optical properties of the dye and/or the insertion efficiency, indicating that the fluorescence emission of this type of self-inserting dye may not be reliable for determining size or size distribution of BNPs with complex lipid compositions.
Subject headings
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology -- Nano-technology (hsv//eng)
- NATURVETENSKAP -- Fysik -- Den kondenserade materiens fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Condensed Matter Physics (hsv//eng)
Keyword
- microfluidics
- Fluorescence
- Nanoparticles
- Microscopy
- Membranes
- Vesicles
- Lipids
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
-
Langmuir
(Search for host publication in LIBRIS)
To the university's database
- By the author/editor
-
Jõemetsa, Silver ...
-
Joyce, Paul, 198 ...
-
Lubart, Quentin, ...
-
Mapar, Mokhtar, ...
-
Celauro, Emanuel ...
-
Agnarsson, Björn ...
-
show more...
-
Block, Stephan, ...
-
Bally, Marta, 19 ...
-
Esbjörner Winter ...
-
Jeffries, Gavin, ...
-
Höök, Fredrik, 1 ...
-
Esbjorner, Elin ...
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Chemical Science ...
-
and Materials Chemis ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Physical Science ...
-
and Other Physics To ...
-
- ENGINEERING AND TECHNOLOGY
-
ENGINEERING AND ...
-
and Nano technology
-
and Nano technology
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Physical Science ...
-
and Condensed Matter ...
- Articles in the publication
-
Langmuir
- By the university
-
Chalmers University of Technology
-
Umeå University