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Second harmonic gen...
Second harmonic generation for collagen I characterization in rectal cancer patients with and without preoperative radiotherapy
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- Blockhuys, Stephanie, 1983 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Agarwal, Nisha Rani, 1987 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Hildesjo, C. (författare)
- Linköpings universitet,Linköping University,Universitetssjukhuset i Linköping,Linköping University Hospital
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- Jarlsfelt, I. (författare)
- Länssjukhuset Ryhov,County Hospital Ryhov
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- Wittung Stafshede, Pernilla, 1968 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Sun, X. F. (författare)
- Linköpings universitet,Linköping University
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(creator_code:org_t)
- SPIE-Intl Soc Optical Eng, 2017
- 2017
- Engelska.
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Ingår i: Journal of Biomedical Optics. - : SPIE-Intl Soc Optical Eng. - 1560-2281 .- 1083-3668. ; 22:10
- Relaterad länk:
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http://publications.... (primary) (free)
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https://www.spiedigi...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Rectal cancer is treated with preoperative radiotherapy (RT) to downstage the tumor, reduce local recurrence, and improve patient survival. Still, the treatment outcome varies significantly and new biomarkers are desired. Collagen I (Col-I) is a potential biomarker, which can be visualized label-free by second harmonic generation (SHG). Here, we used SHG to identify Col-I changes induced by RT in surgical tissue, with the aim to evaluate the clinical significance of RT-induced Col-I changes. First, we established a procedure for quantitative evaluation of Col-I by SHG in CDX2-stained tissue sections. Next, we evaluated Col-I properties in material from 31 non-RT and 29 RT rectal cancer patients. We discovered that the Col-I intensity and anisotropy were higher in the tumor invasive margin than in the inner tumor and normal mucosa, and RT increased and decreased the intensity in inner tumor and normal mucosa, respectively. Furthermore, higher Col-I intensity in the inner tumor was related to increased distant recurrence in the non-RT group but to longer survival in the RT group. In conclusion, we present a new application of SHG for quantitative analysis of Col-I in surgical material, and the first data suggest Col-I intensity as a putative prognostic biomarker in rectal cancer.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- collagen I
- rectal cancer
- radiotherapy
- prognosis
- second harmonic generation
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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