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Affinity Capturing ...
Affinity Capturing and Surface Enrichment of a Membrane Protein Embedded in a Continuous Supported Lipid Bilayer
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- Gunnarsson, Anders, 1981 (författare)
- AstraZeneca AB
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- Simonsson Nyström, Lisa, 1982 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Burazerovic, Sabina, 1976 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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visa fler...
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- Gunnarsson, J. (författare)
- AstraZeneca AB
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- Snijder, Arjan, 1971 (författare)
- AstraZeneca AB
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- Geschwindner, S. (författare)
- AstraZeneca AB
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- Höök, Fredrik, 1966 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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visa färre...
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(creator_code:org_t)
- 2016-08-22
- 2016
- Engelska.
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Ingår i: ChemistryOpen. - : Wiley. - 2191-1363. ; 5:5, s. 445-449
- Relaterad länk:
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http://publications.... (primary) (free)
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https://onlinelibrar...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Investigations of ligand-binding kinetics to membrane proteins are hampered by their poor stability and low expression levels, which often translates into sensitivity-related limitations impaired by low signal-to-noise ratios. Inspired by affinity capturing of water-soluble proteins, which utilizes water as the mobile phase, we demonstrate affinity capturing and local enrichment of membrane proteins by using a fluid lipid bilayer as the mobile phase. Specific membrane-protein capturing and enrichment in a microfluidic channel was accomplished by immobilizing a synthesized trivalent nitrilotriacetic acid (tris-NTA)-biotin conjugate. A polymer-supported lipid bilayer containing His(6)-tagged b-secretase (BACE) was subsequently laterally moved over the capture region by using a hydrodynamic flow. Specific enrichment of His(6)-BACE in the Ni2+-NTA-modified region of the substrate resulted in a stationary three-fold increase in surface coverage, and an accompanied increase in ligand-binding response.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
Nyckelord
- affinity purification
- membrane proteins
- supported lipid bilayer
- surface chemistry
- His-tag
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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