An LC-QToF MS based method for untargeted metabolomics of human fecal samples

Cheng, Ken,1987Chalmers tekniska högskola,Chalmers University of Technology (författare)

Introduction: Consensus in sample preparation for untargeted human fecal metabolomics is lacking. Objectives: To obtain sample preparation with broad metabolite coverage for high-throughput LC–MS. Methods: Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality. Results: Methanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both fresh frozen and lyophilized samples. Lyophilization did not affect SCFA and is recommended because of convenience in normalizing to dry matter. Conclusion: The suggested sample preparation is simple, efficient and suitable for large-scale human fecal metabolomics.

Brunius, Carl,1974Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)carlbru (författare)
Fristedt, Rikard,1978Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)rikfri (författare)
Landberg, Rikard,1981Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)riklan (författare)
Chalmers tekniska högskola (creator_code:org_t)

Ingår i:Metabolomics: Springer Science and Business Media LLC16:41573-38821573-3890

Av författaren/redakt...: Cheng, Ken, 1987; Brunius, Carl, 1 ...; Fristedt, Rikard ...; Landberg, Rikard ...

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