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Role of Zero-Order ...
Role of Zero-Order Loop in Protein Unfolding Case Study with Apoazurin
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- Homouz, Dirar M. (author)
- Khalifa University of Science and Technology,University of Houston
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- Zegarra, Fabio (author)
- University of Houston
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- Wittung Stafshede, Pernilla, 1968 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Cheung, Margaret S. (author)
- Rice University,University of Houston
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(creator_code:org_t)
- Elsevier BV, 2020
- 2020
- English.
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In: Biophysical Journal. - : Elsevier BV. - 0006-3495 .- 1542-0086. ; 118:3, Supplement 1, s. 197A-197A
- Related links:
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http://www.cell.com/...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Pseudomonas aeruginosa apoazurin (apo, without the copper cofactor) is a two-state folder that has a single disulfide bond between residues 3 and 26. This bond covalently connects the N-termini of beta-strands 1 and 3; thereby it creates a zero-order loop. The loop restricts the conformational space for the apoazurin polypeptide. In order to understand the role played by the zero-order loop, we used molecular dynamics (MD) simulations to compare two variants of apoazurin; one variant called “loop” which contained the disulfide and another called “open” in which the disulfide bond between residues 3 and 26 was removed. MD simulations were performed to probe the unfolding pathway and stability of the two apoazurin variants at different urea concentrations and temperatures. Our results show that the folded structure apoazurin is somewhat more stable due to the presence of the disulfide bond. However, the disulfide bond plays a prominent role in the apoazurin unfolding mechanism: we find that it changes both the folding-transition state and the unfolded-state ensemble of conformations.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Keyword
- Apoazurin
- zero-order loop
Publication and Content Type
- kon (subject category)
- ref (subject category)
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