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- Homouz, Dirar M.Khalifa University of Science and Technology,University of Houston (author)
Role of Zero-Order Loop in Protein Unfolding Case Study with Apoazurin
- Article/chapterEnglish2020
Publisher, publication year, extent ...
- Elsevier BV,2020
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- LIBRIS-ID:oai:research.chalmers.se:6c9b388e-f014-4924-b26d-0fb4b1dc3fe1
- https://doi.org/10.1016/j.bpj.2019.11.1191DOI
- https://research.chalmers.se/publication/516034URI
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- Language:English
- Summary in:English
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- SwePubSwePub
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- Pseudomonas aeruginosa apoazurin (apo, without the copper cofactor) is a two-state folder that has a single disulfide bond between residues 3 and 26. This bond covalently connects the N-termini of beta-strands 1 and 3; thereby it creates a zero-order loop. The loop restricts the conformational space for the apoazurin polypeptide. In order to understand the role played by the zero-order loop, we used molecular dynamics (MD) simulations to compare two variants of apoazurin; one variant called “loop” which contained the disulfide and another called “open” in which the disulfide bond between residues 3 and 26 was removed. MD simulations were performed to probe the unfolding pathway and stability of the two apoazurin variants at different urea concentrations and temperatures. Our results show that the folded structure apoazurin is somewhat more stable due to the presence of the disulfide bond. However, the disulfide bond plays a prominent role in the apoazurin unfolding mechanism: we find that it changes both the folding-transition state and the unfolded-state ensemble of conformations.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Fysiologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Physiology hsv//eng
- NATURVETENSKAP Fysik Annan fysik hsv//swe
- NATURAL SCIENCES Physical Sciences Other Physics Topics hsv//eng
- NATURVETENSKAP Biologi Biofysik hsv//swe
- NATURAL SCIENCES Biological Sciences Biophysics hsv//eng
- Apoazurin
- zero-order loop
Added entries (persons, corporate bodies, meetings, titles ...)
- Zegarra, FabioUniversity of Houston (author)
- Wittung Stafshede, Pernilla,1968Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)perwit (author)
- Cheung, Margaret S.Rice University,University of Houston (author)
- Khalifa University of Science and TechnologyUniversity of Houston (creator_code:org_t)
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- In:Biophysical Journal: Elsevier BV118:3, Supplement 1, s. 197A-197A0006-34951542-0086
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