Injectable In Situ Crosslinking Hydrogel for Autologous Fat Grafting

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MARC

Oskarsdotter, Kristin,1995Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery (author)

Injectable In Situ Crosslinking Hydrogel for Autologous Fat Grafting

Article/chapterEnglish2023

Publisher, publication year, extent ...

2023
electronicrdacarrier

Numbers

LIBRIS-ID:oai:research.chalmers.se:6fba5698-c9a6-4274-924e-009e19ef70ca
https://doi.org/10.3390/gels9100813DOI
https://research.chalmers.se/publication/538296URI
https://gup.ub.gu.se/publication/331600URI

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Language:English
Summary in:English

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SwePubSwePub

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Subject category:art swepub-publicationtype
Subject category:ref swepub-contenttype

Notes

Autologous fat grafting is hampered by unpredictable outcomes due to high tissue resorption. Hydrogels based on enzymatically pretreated tunicate nanocellulose (ETC) and alginate (ALG) are biocompatible, safe, and present physiochemical properties capable of promoting cell survival. Here, we compared in situ and ex situ crosslinking of ETC/ALG hydrogels combined with lipoaspirate human adipose tissue (LAT) to generate an injectable formulation capable of retaining dimensional stability in vivo. We performed in situ crosslinking using two different approaches; inducing Ca2+ release from CaCO3 microparticles (CMPs) and physiologically available Ca2+ in vivo. Additionally, we generated ex situ-crosslinked, 3D-bioprinted hydrogel-fat grafts. We found that in vitro optimization generated a CMP-crosslinking system with comparable stiffness to ex situ-crosslinked gels. Comparison of outcomes following in vivo injection of each respective crosslinked hydrogel revealed that after 30 days, in situ crosslinking generated fat grafts with less shape retention than 3D-bioprinted constructs that had undergone ex situ crosslinking. However, CMP addition improved fat-cell distribution and cell survival relative to grafts dependent on physiological Ca2+ alone. These findings suggested that in situ crosslinking using CMP might promote the dimensional stability of injectable fat-hydrogel grafts, although 3D bioprinting with ex situ crosslinking more effectively ensured proper shape stability in vivo.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Kirurgi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Surgery hsv//eng
NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
NATURVETENSKAP Kemi Polymerkemi hsv//swe
NATURAL SCIENCES Chemical Sciences Polymer Chemistry hsv//eng
nanocellulose
alginate
in situ crosslinking
3D bioprinting
soft tissue reconstruction
soft tissue reconstruction
in situ crosslinking
3D bioprinting
nanocellulose
alginate

Added entries (persons, corporate bodies, meetings, titles ...)

Nordgård, Catherine T.Norges teknisk-naturvitenskapelige universitet (NTNU),Norwegian University of Science and Technology (NTNU) (author)
Apelgren, PeterGothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery(Swepub:gu)xapelp (author)
Säljö, Karin,1981Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery(Swepub:gu)xsaljk (author)
Solbu, Anita A.Norges teknisk-naturvitenskapelige universitet (NTNU),Norwegian University of Science and Technology (NTNU) (author)
Eliasson, EdwinSahlgrenska universitetssjukhuset,Sahlgrenska University Hospital (author)
Sämfors, Sanna,1987Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)samfors (author)
Sætrang, Henriette E.M. (author)
Asdahl, Lise Cathrine (author)
Thompson, Eric M.Universitetet i Bergen,University of Bergen (author)
Troedsson, Christofer (author)
Simonsson, Stina,1969Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital (author)
Strand, Berit LøkensgardNorges teknisk-naturvitenskapelige universitet (NTNU),Norwegian University of Science and Technology (NTNU) (author)
Gatenholm, Paul,1956(Swepub:cth)gatenh (author)
Kölby, Lars,1963Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery(Swepub:gu)xkolbl (author)
Göteborgs universitetInstitutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi (creator_code:org_t)

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Surgery

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Polymer Chemistr ...

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