Thermodynamic Aspects of DNA Nanoconstruct Stability and Design

Sandin, Peter,1977Chalmers tekniska högskola,Chalmers University of Technology (författare)

In the present study, we use the fluorescent DNA base analog tCå to investigate the thermal stability of a small DNA hexagon and the thermodynamie factors that govern the formation of such a structure. The DNA molecule is becoming increasingly popular as a material for bottom-up construction of nanostructures; however, relatively little attention has been given to the thermodynamics of such biomacromolecule-based constructs. With the goal of increasing information density and structural complexity, the size of the nanoarchitectures decreases and, more importantly, the fine structure is becoming more detailed. In this process the thermal stability and formation of unwanted byproducts will become critical features to consider in the design and assembly of such structures. Using tCå as a fluorescent probe in fluorescence monitored DNA melting allows for individually observing the denaturing of each of the six 10-mer sides in the pseudohexagonal multicomponent system. Experimental results demonstrate that the ring-opening of the cyclized hexamer is virtually exclusive to one side and that the stability of this side is increased as a result of the cyclization. Moreover, a theoretical model describing the formation and melting of the nanostructure is presented. The results show that the cyclized structure is thermodynamically favored over linear polymeric structures under the conditions and concentrations used for the self-assembly. © 2009 American Chemical Society.

Tumpane, John,1983Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)tumpane (författare)
Börjesson, Karl,1982Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)kb01boka (författare)
Wilhelmsson, Marcus,1974Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)mawi (författare)
Brown, T.University of Southampton (författare)
Nordén, Bengt,1945Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)norden (författare)
Albinsson, Bo,1963Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)balb (författare)
Lincoln, Per,1958Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)lincoln (författare)
Chalmers tekniska högskolaUniversity of Southampton (creator_code:org_t)

Ingår i:Journal of Physical Chemistry C: American Chemical Society (ACS)113:15, s. 5941-59461932-74471932-7455

Av författaren/redakt...: Sandin, Peter, 1 ...; Tumpane, John, 1 ...; Börjesson, Karl, ...; Wilhelmsson, Mar ...; Brown, T.; Nordén, Bengt, 1 ...; visa fler...; Albinsson, Bo, 1 ...; Lincoln, Per, 19 ...; visa färre...

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