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  • Hedsten, Karin,1964Chalmers tekniska högskola,Chalmers University of Technology (author)

Optical Label-Free Nanoplasmonic Biosensing Using a Vertical-Cavity Surface-Emitting Laser and Charge-Coupled Device

  • Article/chapterEnglish2010

Publisher, publication year, extent ...

  • 2010-01-19
  • American Chemical Society (ACS),2010

Numbers

  • LIBRIS-ID:oai:research.chalmers.se:795f1eff-b27e-4188-8555-e26a29e2c89b
  • https://research.chalmers.se/publication/112160URI
  • https://doi.org/10.1021/ac9025169DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • We present a compact platform for biochemosensing based on the combination of a vertical-cavity surface- emitting laser (VCSEL) light source, microelectrome- chanical systems (MEMS)-based microoptics, a specially designed nanoplasmonic sensing chip, and charge- coupled device (CCD) detector. The platform does not require any spectral analyzer for signal evaluation, show- ing good promise for facile integration, neither does it use any microscope setup for the signal collection or imaging. The analytical capabilities of the developed biochemosens- ing platform are demonstrated by evaluation of the protein-substrate (biotinylated bovine serum albumin- gold) and the protein-protein (biotin-NeutrAvidin) bind- ing kinetics, which is further compared to detection based on conventional optical extinction spectroscopy. The instrument is able to detect low femtomoles of adsorbed proteins with the limit of detection comparable to the state-of-the-art research and commercial optical label-free biochemosensors.

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Added entries (persons, corporate bodies, meetings, titles ...)

  • Fonollosa, JordiChalmers tekniska högskola,Chalmers University of Technology (author)
  • Enoksson, Peter,1957Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)enok (author)
  • Modh, Peter,1968Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)modh (author)
  • Bengtsson, Jörgen,1968Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)jorgenb (author)
  • Sutherland, Duncan,1970Universitat de Barcelona,University of Barcelona(Swepub:cth)duncan (author)
  • Dmitriev, Alexander,1975Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)alexd (author)
  • Chalmers tekniska högskolaUniversitat de Barcelona (creator_code:org_t)

Related titles

  • In:Analytical Chemistry: American Chemical Society (ACS)82:4, s. 1535-15390003-27001520-6882

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