Crystallization and preliminary crystallographic analysis of the NAD(H)-binding domain of Escherichia coli transhydrogenase

Oswald, ChristineChalmers tekniska högskola,Chalmers University of Technology (author)

Transhydrogenase is a proton-pumping membrane protein that is required for the cellular regeneration of NADPH. The NAD(H)-binding domain (domain I) of transhydrogenase from Escherichia coli was crystallized using the hanging-drop vapour-diffusion technique at room temperature. The crystals, which were grown from PEG 4000 and ammonium acetate in citrate buffer, belong to the triclinic space group P1, with unit-cell parameters a = 38.8, b = 66.8, c = 76.4 Å, α = 67.5, β = 80.8, γ = 81.5°. X-ray diffraction data were collected to 1.9 Å resolution using synchrotron radiation. The crystals contain one dimer of transhydrogenase domain I per asymmetric unit. © 2004 International Union of Crystallography. Printed in Denmark - all rights reserved.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
NATURVETENSKAP Biologi Biofysik hsv//swe
NATURAL SCIENCES Biological Sciences Biophysics hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Medicinsk bioteknologi hsv//swe
MEDICAL AND HEALTH SCIENCES Medical Biotechnology Medical Biotechnology hsv//eng
NATURVETENSKAP Kemi hsv//swe
NATURAL SCIENCES Chemical Sciences hsv//eng
rhodospirillum-rubrum
refinement
complex
crystal-structure
resolution
proton-translocating transhydrogenase
nicotinamide nucleotide transhydrogenase
nadp(h)-binding component diii
catalytic-properties
site
proton-translocating transhydrogenase
nicotinamide nucleotide transhydrogenase
nadp(h)-binding component diii
rhodospirillum-rubrum
catalytic-properties
crystal-structure
refinement
resolution
complex
site

Johansson, TomasChalmers tekniska högskola,Chalmers University of Technology (author)
Törnroth-Horsefield, Susanna,1973Chalmers tekniska högskola,Chalmers University of Technology(Swepub:gu)xtorsu (author)
Ökvist, M.Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry (author)
Krengel, Ute,1964Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)mbtuk (author)
Chalmers tekniska högskolaInstitutionen för kemi (creator_code:org_t)

In:Acta Crystallographica Section D: Biological Crystallography60:4, s. 743-7451399-00470907-4449

By the author/editor: Oswald, Christin ...; Johansson, Tomas; Törnroth-Horsefi ...; Ökvist, M.; Krengel, Ute, 19 ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biophysics

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Medical Biotechn ...

By the university: Chalmers University of Technology; University of Gothenburg

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