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Exploring carbon so...
Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based a pproaches
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- Braam, Svenja, 1989 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper,Department of Mathematical Sciences,Chalmers tekniska högskola,Chalmers University of Technology
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- Tripodi, Farida (författare)
- Universita' degli Studi di Milano-Bicocca,University of Milano-Bicocca
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- Österberg, Linnea, 1992 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper,Department of Mathematical Sciences,Chalmers tekniska högskola,Chalmers University of Technology,Universita' degli Studi di Milano-Bicocca,University of Milano-Bicocca
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- Persson, Sebastian (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper,Department of Mathematical Sciences,Chalmers tekniska högskola,Chalmers University of Technology
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- Welkenhuysen, Niek, 1988 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper,Department of Mathematical Sciences,Chalmers tekniska högskola,Chalmers University of Technology,Universita' degli Studi di Milano-Bicocca,University of Milano-Bicocca
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- Coccetti, Paola (författare)
- Universita' degli Studi di Milano-Bicocca,University of Milano-Bicocca
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- Cvijovic, Marija, 1977 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper,Department of Mathematical Sciences,Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2024
- 2024
- Engelska.
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Ingår i: MICROBIAL CELL. - 2311-2638. ; 11:1, s. 143-154
- Relaterad länk:
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https://gup.ub.gu.se...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- The AMPK/SNF1 pathway governs energy balance in eukaryotic cells, notably influencing glucose de-repression. In S. cerevisiae , Snf1 is phosphorylated and hence activated upon glucose depletion. This activation is required but is not sufficient for mediating glucose de-repression, indicating further glucosedependent regulation mechanisms. Employing fluorescence recovery after photobleaching (FRAP) in conjunction with non -linear mixed effects modelling, we explore the spatial dynamics of Snf1 as well as the relationship between Snf1 phosphorylation and its target Mig1 controlled by hexose sugars. Our results suggest that inactivation of Snf1 modulates Mig1 localization and that the kinetic of Snf1 localization to the nucleus is modulated by the presence of non -fermentable carbon sources. Our data offer insight into the true complexity of regulation of this central signaling pathway in orchestrating cellular responses to fluctuating environmental cues. These insights not only expand our understanding of glucose homeostasis but also pave the way for further studies evaluating the importance of Snf1 localization in relation to its phosphorylation state and regulation of downstream targets.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- yeast
- nutrient signaling
- Snf1/Mig1 network
- nuclear localization
- fluorescence recovery after photobleaching
- nonlinear mixed effect model
- nuclear localization
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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