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GLIOBLASTOMA GROWTH...
GLIOBLASTOMA GROWTH IS SHAPED BY INVASION ROUTE-SPECIFIC FUNCTIONAL SIGNATURES
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- Krona, Cecilia (author)
- Uppsala universitet,Uppsala University
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- Rosen, Emil (author)
- Uppsala universitet,Uppsala University
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- Kundu, Soumi (author)
- Uppsala universitet,Uppsala University
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show more...
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- Olausson, Karl Holmberg (author)
- Uppsala universitet,Uppsala University
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- Larsson, Ida (author)
- Uppsala universitet,Uppsala University
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- Elfineh, Ludmila (author)
- Uppsala universitet,Uppsala University
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- Doroszko, Milena (author)
- Uppsala universitet,Uppsala University
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- Elgendy, Ramy (author)
- Uppsala universitet,Uppsala University
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- Wickstrom, Johan (author)
- Uppsala universitet,Uppsala University
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- Jörnsten, Rebecka, 1971 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Nelander, Sven (author)
- Uppsala universitet,Uppsala University
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show less...
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(creator_code:org_t)
- 2023
- 2023
- English.
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In: Neuro-Oncology. - 1523-5866 .- 1522-8517. ; 25:Supplement: 5, MODL-16
- Related links:
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https://research.cha...
Abstract
Subject headings
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- One of the defining features of glioblastomas (GBMs) is the capacity for invasive growth along multiple anatomical pathways in the brain. GBM is well-studied on a genetic and molecular level, but clinically relevant and experimentally tractable models of invasive growth are largely lacking. Here, we report an integrated study of patient-matched information, genomic- and molecular profiles with growth in mouse brains to expose treatments and biomarkers associated with glioblastoma invasion and recurrence. In total, 64 patient-derived cell lines (PDCLs) were injected into the striatum of n ≥ 4 mice each. The 45 tumor-forming PDCLs were each scored for 10 distinct growth characteristics (n = 182 mice). The repertoire of phenotypes was highly divergent, and our material included clear cases of perivascular route invasion, white matter route invasion, perineuronal satellitosis, and gliosarcoma. We explored if cellular pathways, monitored by RNA-sequencing, could account for these differences. GSEA highlighted a positive enrichment for highly proliferative proneural tumors characterized by Notch activation, neuronal signaling, and epigenetic gene regulatory programs in the tumor-initiating lines. Transcriptional signatures were also strongly predictive of route-specific invasion. Diffuse invasion was predominantly seen in classical-subtype PDCLs with astrocytic or outer radial glia-like signatures. Proneural PDCLs, in turn, grew as solid tumors with an invasive peripheral region around vasculature, and mesenchymal tumors were more demarcated. To explore the therapeutic implications of our findings, we used our data-driven method (TargetTranslator, Nat Comm 2020) to predict the drug vulnerabilities of different types of invasive glioblastoma. Defined GBM tumors with perivascular invasion are characterized by increased IGFR1, MAPK/ERK, PI3K/AKT/mTOR, and JAK2 signaling. Diffusively growing GBM tumors, on the other hand, depend more on Wnt/β-catenin signaling, neuronal signaling, and active inflammatory response. Using a sphere invasion assay, we confirm that targeting both PI3K- and Wnt signaling selectively reduces glioblastoma invasion, highlighting their therapeutic potential.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Publication and Content Type
- kon (subject category)
- vet (subject category)
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To the university's database
- By the author/editor
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Krona, Cecilia
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Rosen, Emil
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Kundu, Soumi
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Olausson, Karl H ...
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Larsson, Ida
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Elfineh, Ludmila
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show more...
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Doroszko, Milena
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Elgendy, Ramy
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Wickstrom, Johan
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Jörnsten, Rebeck ...
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Nelander, Sven
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
- Articles in the publication
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Neuro-Oncology
- By the university
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Chalmers University of Technology