Asymmetric cationic liposomes designed for heat-activated association with cells

Jing, Yujia,1985Chalmers tekniska högskola,Chalmers University of Technology (författare)

Improved anticancer drugs and drug carriers are needed in combination therapies, such as hyperthermia-assisted chemotherapy. Liposomal drug carriers with advanced functions are attractive candidates for targeted accumulation and drug release in response to heat stimulus. We report on the design of liposomes with a heat-activated surface function. Our design is based on asymmetric lipid membranes with a defined gel to liquid-crystalline phase-transition temperature around 41 °C. Asymmetry between the inner and the outer membrane leaflets was generated through selective PEGylation of cationic lipids in the outer membrane leaflet. In a physiological buffer, the PEGylated asymmetric liposomes had a neutral zeta potential and did not bind to planar anionic model membranes. In contrast, following upon heat-activation, binding of liposomes to the model membranes occurred. Release of a hydrophilic dye encapsulated in the asymmetric liposomes occurred at 40 °C. Enhanced uptake of the asymmetric liposomes by hypopharyngeal carcinoma cells (FaDu cells) was observed when hyperthermia was applied compared to experiments performed at 37 °C. These results show the potential of asymmetric liposomes for localized delivery of drugs into cells in response to (external) temperature stimulus.

Danielsson, Anna,1973Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för onkologi,Sahlgrenska Cancer Center,Institute of Clinical Sciences, Department of Oncology(Swepub:gu)xdanne (författare)
Dobsicek Trefna, Hana,1979Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)hanatre (författare)
Persson, Mikael,1959Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)elfmp (författare)
Svedhem, Sofia,1970Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)sofsv (författare)
Chalmers tekniska högskolaInstitutionen för kliniska vetenskaper, Avdelningen för onkologi (creator_code:org_t)

Ingår i:Colloids and Surfaces B: Biointerfaces: Elsevier BV151, s. 112-1180927-77651873-4367

Av författaren/redakt...: Jing, Yujia, 198 ...; Danielsson, Anna ...; Dobsicek Trefna, ...; Persson, Mikael, ...; Svedhem, Sofia, ...

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Cancer och onkol ...

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