Characterization of CYP82 genes involved in the biosynthesis of structurally diverse benzylisoquinoline alkaloids in Corydalis yanhusuo

Benzylisoquinoline alkaloids (BIAs) represent a significant class of secondary metabolites with crucial roles in plant physiology and substantial potential for clinical applications. CYP82 genes are involved in the formation and modification of various BIA skeletons, contributing to the structural diversity of compounds. In this study, Corydalis yanhusuo, a traditional Chinese medicine rich in BIAs, was investigated to identify the catalytic function of CYP82s during BIA formation. Specifically, 20 CyCYP82-encoding genes were cloned, and their functions were identified in vitro. Ten of these CyCYP82s were observed to catalyze hydroxylation, leading to the formation of protopine and benzophenanthridine scaffolds. Furthermore, the correlation between BIA accumulation and the expression of CyCYP82s in different tissues of C. yanhusuo was assessed their. The identification and characterization of CyCYP82s provide novel genetic elements that can advance the synthetic biology of BIA compounds such as protopine and benzophenanthridine, and offer insights into the biosynthesis of BIAs with diverse structures in C. yanhusuo.

Ma, YingChina Academy of Chinese Medical Sciences (författare)
Bu, JunlingChina Academy of Chinese Medical Sciences (författare)
Lian, ConglongHenan University of Chinese Medicine (författare)
Ma, RuiHenan University of Chinese Medicine (författare)
Li, QishuangChina Academy of Chinese Medical Sciences (författare)
Jiao, Xiang,1990Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)xiangj (författare)
Hu, ZhiminChina Academy of Chinese Medical Sciences (författare)
Chen, Yun,1978Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)yunc (författare)
Chen, SuiqingHenan University of Chinese Medicine (författare)
Guo, JuanChina Academy of Chinese Medical Sciences (författare)
Huang, LuqiChina Academy of Chinese Medical Sciences (författare)
Henan University of Chinese MedicineChina Academy of Chinese Medical Sciences (creator_code:org_t)

Av författaren/redakt...: Liu, Xiuyu; Ma, Ying; Bu, Junling; Lian, Conglong; Ma, Rui; Li, Qishuang; visa fler...; Jiao, Xiang, 199 ...; Hu, Zhimin; Chen, Yun, 1978; Chen, Suiqing; Guo, Juan; Huang, Luqi; visa färre...

Om ämnet

TEKNIK OCH TEKNOLOGIER: TEKNIK OCH TEKNO ...; och Industriell biot ...; och Biokatalys och e ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.