Search: L773:1096 7176 OR L773:1096 7184 >
Adaptively evolved ...
Adaptively evolved yeast mutants on galactose show trade-offs in carbon utilization on glucose
-
- Hong, Kuk-ki, 1976 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Nielsen, Jens B, 1962 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
-
(creator_code:org_t)
- Elsevier BV, 2013
- 2013
- English.
-
In: Metabolic Engineering. - : Elsevier BV. - 1096-7176 .- 1096-7184. ; 16:1, s. 78-86
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://research.cha...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Adaptive evolution offers many opportunities in metabolic engineering; however, several constraints still exist as evolutionary trade-offs may impose collateral cost to obtain new traits. The application of adaptive evolution for strains development could be further improved by elucidating the molecular mechanisms. In this study, adaptively evolved yeast mutants with improved galactose utilization ability showed impaired glucose utilization. The molecular genetic basis of this trade-off was investigated using a systems biology approach. Transcriptional and metabolic changes resulting from the improvement of galactose utilization were found maintained during growth on glucose. Moreover, glucose repression related genes showed conserved expression patterns during growth on both sugars. Mutations in the RAS2 gene that were identified as beneficial for galactose utilization in evolved mutants exhibited significant correlation with attenuation of glucose utilization. These results indicate that antagonistic pleiotropy is the dominant mechanism in the observed trade-off, and it is likely realized by changes in glucose signaling.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- adaptation
- Systems biology
- saccharomyces-cerevisiae
- escherichia-coli
- Metabolic engineering
- Galactose metabolism
- evolution
- Evolutionary trade-off
- populations
- Glucose
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database