In vitro analysis of α-synuclein amyloid formation and cross-reactivity

Horvath, Istvan,1979Chalmers tekniska högskola,Chalmers University of Technology (författare)

In vitro time-resolved characterization of protein aggregation into amyloid fibers and the effects of other proteins on the aggregation process are fundamentally important measurements to obtain a better understanding of the mechanisms contributing to neurodegeneration, as well as other diseases involving amyloid formation. Here, we describe how to perform in vitro aggregation experiments with α-synuclein, the amyloidogenic protein involved in Parkinson’s disease, including how to assess the starting material, useful experimental/instrumental conditions, as well as how to set up cross-seeding and co-aggregation experiments. The high variability of data reported for in vitro α-synuclein amyloid formation may in part be explained by experimental differences.

Rocha, Sandra,1975Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)rochas (författare)
Wittung Stafshede, Pernilla,1968Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)perwit (författare)
Chalmers tekniska högskola (creator_code:org_t)

Ingår i:Methods in Molecular BiologyNew York, NY : Springer New York, s. 73-831940-60291064-3745

Av författaren/redakt...: Horvath, Istvan, ...; Rocha, Sandra, 1 ...; Wittung Stafshed ...

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinsk biotek ...; och Medicinsk biotek ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.