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- Berglund, E. C.Göteborgs universitet,University of Gothenburg (author)
Freeze-Drying as Sample Preparation for Micellar Electrokinetic Capillary Chromatography-Electrochemical Separations of Neurochemicals in Drosophila Brains
- Article/chapterEnglish2013
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- 2013-02-22
- American Chemical Society (ACS),2013
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- LIBRIS-ID:oai:research.chalmers.se:9c06cf37-399f-46e2-a0a3-5cff9308f4bb
- https://doi.org/10.1021/ac303377xDOI
- https://research.chalmers.se/publication/176816URI
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- Language:English
- Summary in:English
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- Micellar electrokinetic capillary chromatography with electrochemical detection has been used to quantify biogenic amines in freeze-dried brains of Drosophila melanogaster. Freeze-drying samples offers a way to preserve the biological sample while making dissection of these tiny samples easier and faster. Fly samples were extracted in cold acetone and dried in a rotary evaporator. Extraction and drying times were optimized in order to avoid contamination by red pigment from the fly eyes and still have intact brain structures. Single freeze-dried fly brain samples were found to produce representative electropherograms as a single hand-dissected brain sample. With utilization of the faster dissection time that freeze-drying affords, the number of brains in a fixed homogenate volume can be increased to concentrate the sample. Thus, concentrated brain samples containing five or fifteen preserved brains were analyzed for their neurotransmitter content, and four analytes; N-acetyloctopamine, N-acetylserotonin, N-acetyltyramine, and N-acetyldopamine were found to correspond well with previously reported values.
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- Kuklinski, N. J.Pennsylvania State University (author)
- Karagunduz, E.Göteborgs universitet,University of Gothenburg (author)
- Ucar, Kubra,1986Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)kobra (author)
- Hanrieder, Jörg,1980Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)hjorg (author)
- Ewing, Andrew,1957Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)andrewe (author)
- Göteborgs universitetPennsylvania State University (creator_code:org_t)
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- In:Analytical Chemistry: American Chemical Society (ACS)85:5, s. 2841-28460003-27001520-6882
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