Search: (WFRF:(Kim H. S.)) srt2:(1991-1994) >
PNA HYBRIDIZES TO C...
PNA HYBRIDIZES TO COMPLEMENTARY OLIGONUCLEOTIDES OBEYING THE WATSON-CRICK HYDROGEN-BONDING RULES
- Article/chapterEnglish1993
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Springer Science and Business Media LLC,1993
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LIBRIS-ID:oai:research.chalmers.se:a0e398d4-531d-464e-a942-1f71f89e96e1
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https://research.chalmers.se/publication/144347URI
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https://doi.org/10.1038/365566a0DOI
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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DNA ANALOGUES are currently being intensely investigated owing to their potential as gene-targeted drugs1-3. Furthermore, their properties and interaction with DNA and RNA could provide a better understanding of the structural features of natural DNA that determine its unique chemical, biological and genetic properties3,4. We recently designed a DNA analogue, PNA, in which the backbone is structurally homomorphous with the deoxyribose backbone and consists of N-(2-aminoethyl)glycine units to which the nucleobases are attached5-9. We showed that PNA oligomers containing solely thymine and cytosine can hybridize to complementary oligonucleotides, presumably by forming Watson-Crick-Hoogsteen (PNA)2-DNA triplexes, which are much more stable than the corresponding DNA-DNA duplexes5-7, and bind to double-stranded DNA by strand displacement5,8. We report here that PNA containing all four natural nucleobases hybridizes to complementary oligonucleotides obeying the Watson-Crick base-pairing rules, and thus is a true DNA mimic in terms of base-pair recognition.
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Buchardt, O.
(author)
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Christensen, L.
(author)
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Behrens, C.
(author)
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Freier, S. M.
(author)
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Driver, D. A.
(author)
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Berg, R. H.
(author)
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Kim, Seog K.Chalmers tekniska högskola,Chalmers University of Technology
(author)
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Nordén, Bengt,1945Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)norden
(author)
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Nielsen, P. E.
(author)
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Chalmers tekniska högskola
(creator_code:org_t)
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In:Nature: Springer Science and Business Media LLC365:6446, s. 566-5680028-08361476-4687
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Egholm, M.
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Buchardt, O.
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Christensen, L.
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Behrens, C.
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Freier, S. M.
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Driver, D. A.
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Berg, R. H.
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Kim, Seog K.
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Nordén, Bengt, 1 ...
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Nielsen, P. E.
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- NATURAL SCIENCES
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NATURAL SCIENCES
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Chalmers University of Technology