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Identification and ...
Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis
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- Tiukova, Ievgeniia, 1987 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Møller-Hansen, Iben (author)
- Danmarks Tekniske Universitet,Technical University of Denmark
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- Belew, Zeinu M. (author)
- Köpenhamns universitet,University of Copenhagen
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- Darbani, Behrooz (author)
- Danmarks Tekniske Universitet,Technical University of Denmark
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- Boles, E (author)
- Johann Wolfgang Goethe Universität Frankfurt am Main,Goethe University Frankfurt
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- Nour-Eldin, Hussam H. (author)
- Köpenhamns universitet,University of Copenhagen
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- Linder, Tomas (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences,Sveriges lantbruksuniversitet (SLU),Swedish University of Agricultural Sciences (SLU)
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- Nielsen, Jens B, 1962 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Borodina, I. (author)
- Danmarks Tekniske Universitet,Technical University of Denmark
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(creator_code:org_t)
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- 2019-10-30
- 2019
- English.
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In: FEMS microbiology letters. - : Oxford University Press (OUP). - 0378-1097 .- 1574-6968. ; 366:17
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Abstract
Subject headings
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- The yeast Brettanomyces bruxellensis (syn. Dekkera bruxellensis) is an emerging and undesirable contaminant in industrial low-sugar ethanol fermentations that employ the yeast Saccharomyces cerevisiae. High-affinity glucose import in B. bruxellensis has been proposed to be the mechanism by which this yeast can outcompete S. cerevisiae. The present study describes the characterization of two B. bruxellensis genes (BHT1 and BHT3) believed to encode putative high-affinity glucose transporters. In vitro-generated transcripts of both genes as well as the S. cerevisiae HXT7 high-affinity glucose transporter were injected into Xenopus laevis oocytes and subsequent glucose uptake rates were assayed using 14C-labelled glucose. At 0.1 mM glucose, Bht1p was shown to transport glucose five times faster than Hxt7p. pH affected the rate of glucose transport by Bht1p and Bht3p, indicating an active glucose transport mechanism that involves proton symport. These results suggest a possible role for BHT1 and BHT3 in the competitive ability of B. bruxellensis.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Keyword
- Xenopus laevis
- Brettanomyces bruxellensis
- bioethanol
- yeast
- metabolism
- high-affinity
- glucose transport
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Tiukova, Ievgeni ...
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Møller-Hansen, I ...
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Belew, Zeinu M.
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Darbani, Behrooz
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Boles, E
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Nour-Eldin, Huss ...
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show more...
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Linder, Tomas
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Nielsen, Jens B, ...
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Borodina, I.
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
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and Other Industrial ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Genetics
- Articles in the publication
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FEMS microbiolog ...
- By the university
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Chalmers University of Technology
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Swedish University of Agricultural Sciences