SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:research.chalmers.se:b5ecec64-d51b-4fe4-8660-a3b52d4cdc89"
 

Search: onr:"swepub:oai:research.chalmers.se:b5ecec64-d51b-4fe4-8660-a3b52d4cdc89" > FLOW LINEAR DICHROI...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Hsu, D. S. (author)

FLOW LINEAR DICHROISM AND ELECTRON-MICROSCOPIC ANALYSIS OF PROTEIN-DNA COMPLEXES OF A MUTANT UVRB PROTEIN THAT BINDS TO BUT CANNOT KINK DNA

  • Article/chapterEnglish1994

Publisher, publication year, extent ...

  • Elsevier BV,1994

Numbers

  • LIBRIS-ID:oai:research.chalmers.se:b5ecec64-d51b-4fe4-8660-a3b52d4cdc89
  • https://research.chalmers.se/publication/144031URI
  • https://doi.org/10.1006/jmbi.1994.1541DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • (A)BC excinuclease of Escherichia coli is the enzymatic activity resulting from sequential and partially overlapping actions of UvrA, UvrB, and UvrC protein. UvrA is a molecular matchmaker which promotes the formation of a stable UvrB-damaged DNA complex in which the DNA is kinked by about 130 degrees. The UvrB-DNA complex is then recognized by UvrC) and two incisions are made in the DNA by the joint actions of UvrC and UvrB. A mutant of UvrB (D478A) can be loaded onto the DNA but it does not interact with UvrC to cause a nick 3' to the lesion. Based on the lack of a DNase-I-hypersensitive site in the footprint of the mutant, it was proposed that the lack of incision was due to the inability of the mutant UvrB to kink the DNA. In the current study we have investigated the interaction of the mutant UvrB with DNA using two biophysical methods, flow linear dichroism and electron microscopy. Both methods reveal that the mutant UvrB is unable to bend DNA.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Takahashi, M. (author)
  • Delagoutte, E. (author)
  • Bertrandburggraf, E. (author)
  • Wang, Y. H. (author)
  • Nordén, Bengt,1945Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)norden (author)
  • Fuchs, R. P. P. (author)
  • Griffith, J. (author)
  • Sancar, A. (author)
  • Chalmers tekniska högskola (creator_code:org_t)

Related titles

  • In:Journal of Molecular Biology: Elsevier BV241:5, s. 645-6500022-28361089-8638

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view