Protein profiling of low-density lipoprotein from obese subjects

Karlsson, HelenÖstergötlands Läns Landsting,Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet,Arbets- och miljömedicin,Medicincentrum (author)

Although obesity and high levels of low-density lipoprotein (LDL) are well-known risk factors for cardiovascular disease, the precise role(s) of different LDL constituents in obesity has not been explored. In the present study, we compared the LDL proteome of healthy control adults (body mass index 30). LDL was isolated by density-gradient ultracentrifugation and proteins were separated with 2-D PAGE, quantified, and identified by peptide mass fingerprinting using MALDI-TOF MS. A new LDL-associated protein was identified as transthyretin and found to be significantly more abundant in LDL from the obese subjects. In addition, LDL from the obese subjects contained relatively more alpha(1)-antitrypsin, apo J, apo C-II, than LDL from controls, and also more of an acidic isoform (pI/Mr; 5.2/23 100) of apo A-I. On the other hand, the relative amounts of apo A-IV and the major isoform of apo A-I (pI/Mr; 5.3/23 100) were significantly less in LDL from the obese subjects. Apo E was less and non-sialylated apo C-III more abundant in LDL from obese men than control men, while there were no such differences between LDL from obese and control women. These findings illustrate that obesity is not only associated with increased LDL-cholesterol levels but also with alterations in the LDL protein composition. The presence of transthyretin in LDL from obese subjects may reflect over-nutrition and affect the lipid metabolism in obesity.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
heart-disease
2-DE
Proteomics
cardiovascular-disease
mass-spectrometry
Low-density lipoprotein
Obesity
2-dimensional gel-electrophoresis
acylation-stimulating protein
Transthyretin
human adipocytes
b-containing lipoproteins
apolipoprotein-a-i
apoa-i
retinol-binding-protein
MEDICINE

Mortstedt, H.Linköpings universitet,Linköping University,n/a (author)
Lindqvist, Helen,1977Chalmers tekniska högskola,Chalmers University of Technology,Chalmers(Swepub:cth)hallenst (author)
Tagesson, ChristerÖstergötlands Läns Landsting,Linköpings universitet,Yrkes- och miljömedicin,Hälsouniversitetet,Arbets- och miljömedicin(Swepub:liu)chrta55 (author)
Lindahl, MatsLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:liu)matli17 (author)
Linköpings universitetInstitutionen för klinisk och experimentell medicin (creator_code:org_t)

By the author/editor: Karlsson, Helen; Mortstedt, H.; Lindqvist, Helen ...; Tagesson, Christ ...; Lindahl, Mats

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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