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  • Borja, Gheorghe M.Danmarks Tekniske Universitet,Technical University of Denmark (author)

Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose

  • Article/chapterEnglish2019

Publisher, publication year, extent ...

  • 2019-11-05
  • Springer Science and Business Media LLC,2019
  • electronicrdacarrier

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  • LIBRIS-ID:oai:research.chalmers.se:c248322a-7283-45f7-b7e4-32be2bcd8366
  • https://doi.org/10.1186/s12934-019-1244-4DOI
  • https://research.chalmers.se/publication/513822URI

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  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

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  • Background: Aromatic amino acids and their derivatives are valuable chemicals and are precursors for different industrially compounds. p-Coumaric acid is the main building block for complex secondary metabolites in commercial demand, such as flavonoids and polyphenols. Industrial scale production of this compound from yeast however remains challenging. Results: Using metabolic engineering and a systems biology approach, we developed a Saccharomyces cerevisiae platform strain able to produce 242 mg/L of p-coumaric acid from xylose. The same strain produced only 5.35 mg/L when cultivated with glucose as carbon source. To characterise this platform strain further, transcriptomic analysis was performed, comparing this strain's growth on xylose and glucose, revealing a strong up-regulation of the glyoxylate pathway alongside increased cell wall biosynthesis and unexpectedly a decrease in aromatic amino acid gene expression when xylose was used as carbon source. Conclusions: The resulting S. cerevisiae strain represents a promising platform host for future production of p-coumaric using xylose as a carbon source.

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  • Rodriguez, A.Danmarks Tekniske Universitet,Technical University of Denmark,Köpenhamns universitet,University of Copenhagen (author)
  • Campbell, Kate,1987Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)katec (author)
  • Borodina, I.Danmarks Tekniske Universitet,Technical University of Denmark (author)
  • Chen, Yun,1978Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)yunc (author)
  • Nielsen, Jens B,1962BioInnovation Institute (BII),Köpenhamns universitet,University of Copenhagen,Chalmers tekniska högskola,Chalmers University of Technology,Danmarks Tekniske Universitet,Technical University of Denmark(Swepub:cth)nielsenj (author)
  • Danmarks Tekniske UniversitetKöpenhamns universitet (creator_code:org_t)

Related titles

  • In:Microbial Cell Factories: Springer Science and Business Media LLC18:11475-2859

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