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  • Carlred, Louise M,1985RISE,Medicinteknik (author)

Simultaneous imaging of amyloid-β and lipids in brain tissue using antibody-coupled liposomes and time-of-flight secondary ion mass spectrometry

  • Article/chapterEnglish2014

Publisher, publication year, extent ...

  • 2014-06-30
  • American Chemical Society (ACS),2014

Numbers

  • LIBRIS-ID:oai:research.chalmers.se:cdb181f3-e8f1-4836-a8a4-359f1d78016d
  • https://research.chalmers.se/publication/216681URI
  • https://doi.org/10.1021/ja5019145DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-6744URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:129432332URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • The spatial localization of amyloid-β peptide deposits, the major component of senile plaques in Alzheimer's disease (AD), was mapped in transgenic AD mouse brains using time-of-flight secondary ion mass spectrometry (ToF-SIMS), simultaneously with several endogenous molecules that cannot be mapped using conventional immunohistochemistry imaging, including phospholipids, cholesterol and sulfatides. Whereas the endogenous lipids were detected directly, the amyloid-β deposits, which cannot be detected as intact entities with ToF-SIMS because of extensive ion-induced fragmentation, were identified by specific binding of deuterated liposomes to antibodies directed against amyloid-β. Comparative investigation of the amyloid-β deposits using conventional immunohistochemistry and fluorescence microscopy suggests similar sensitivity but a more surface-confined identification due to the shallow penetration depth of the ToF-SIMS signal. The recorded ToF-SIMS images thus display the localization of lipids and amyloid-β in a narrow (∼10 nm) two-dimensional plane at the tissue surface. As compared to a frozen nontreated tissue sample, the liposome preparation protocol generally increased the signal intensity of endogenous lipids, likely caused by matrix effects associated with the removal of salts, but no severe effects on the tissue integrity and the spatial distribution of lipids were observed with ToF-SIMS or scanning electron microscopy (SEM). This method may provide an important extension to conventional tissue imaging techniques to investigate the complex interplay of different kinds of molecules in neurodegenerative diseases, in the same specimen. However, limitations in target accessibility of the liposomes as well as unspecific binding need further consideration. © 2014 American Chemical Society.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Gunnarsson, Anders,1981Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden; AstraZeneca, Sweden(Swepub:cth)kb01guan (author)
  • Solé-Domènech, S.Weill Cornell Medical College,Lunds universitet,Lund University,Karolinska Institutet,Karolinska Institute, Sweden; Weill Cornell Medical College, USA; Lund University, Sweden (author)
  • Johansson Fast, Björn,1986Karolinska Institutet(Swepub:cth)johabj (author)
  • Vukojević, VladanaKarolinska Institutet (author)
  • Terenius, L.T.Karolinska Institutet (author)
  • Codita, A.Karolinska Institutet (author)
  • Winblad, B.G.Karolinska Institutet (author)
  • Schalling, MartinKarolinska Institutet (author)
  • Höök, Fredrik,1966Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)fredrikh (author)
  • Sjövall, Peter,1961RISE,SP – Sveriges Tekniska Forskningsinstitut / Funktionella material (KMf)(Swepub:ri)PeterS@ri.se (author)
  • RISEMedicinteknik (creator_code:org_t)

Related titles

  • In:Journal of the American Chemical Society: American Chemical Society (ACS)136:28, s. 9973-99811520-51260002-7863

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