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INFOGEST static in ...
INFOGEST static in vitro simulation of gastrointestinal food digestion
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- Brodkorb, A. (author)
- Teagasc - Irish Agriculture and Food Development Authority
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- Egger, L. (author)
- Forschungsanstalt Agroscope Changins-Wadenswil
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- Alminger, Marie, 1957 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Alvito, P. (author)
- Universidade de Aveiro,University of Aveiro
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- Assunção, Ricardo (author)
- Universidade de Aveiro,University of Aveiro
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- Ballance, S. (author)
- Norwegian Institute of Food, Fisheries and Aquaculture Research (Nofima)
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- Bohn, T. (author)
- Luxembourg Institute of Health
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Bourlieu-Lacanal, Claire (author)
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Boutrou, R. (author)
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- Carriere, F. (author)
- Centre national de la recherche scientifique (CNRS)
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Clemente, Alfonso (author)
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- Corredig, M. (author)
- Århus Universitet,Aarhus University
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Dupont, D. (author)
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Dufour, C. (author)
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Edwards, Cathrina (author)
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- Golding, M. (author)
- Massey University
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- Karakaya, S. (author)
- Ege Universitesi,Ege University
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- Kirkhus, B. (author)
- Norwegian Institute of Food, Fisheries and Aquaculture Research (Nofima)
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Le Feunteun, S. (author)
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- Lesmes, U. (author)
- Technion – Israel Institute of Technology
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- Macierzanka, A. (author)
- Politechnika Gdańska,Gdansk University of Technology
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- Mackie, A. (author)
- University of Leeds
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- Martins, Carla (author)
- Universidade de Aveiro,University of Aveiro
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Marze, S. (author)
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- McClements, D. J. (author)
- University of Massachusetts
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Menard, O. (author)
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Minekus, M. (author)
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- Portmann, Reto (author)
- Forschungsanstalt Agroscope Changins-Wadenswil
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- Santos, C. N. (author)
- Nova University of Lisbon, Portugal,Instituto de Biologia Experimental e Tecnologica
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- Souchon, Isabelle (author)
- Université Paris-Saclay,University Paris-Saclay
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- Singh, R. Paul (author)
- University of California
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- Vegarud, G. E. (author)
- Norges miljø- og biovitenskapelige universitet (NMBU),Norwegian University of Life Sciences (NMBU)
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Wickham, M. S. J. (author)
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- Weitschies, W. (author)
- Universität Greifswald,University of Greifswald
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- Recio, I. (author)
- Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM),Institute of Food Science Research (CIAL)
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(creator_code:org_t)
- 2019-03-18
- 2019
- English.
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In: Nature Protocols. - : Springer Science and Business Media LLC. - 1754-2189 .- 1750-2799. ; 14:4, s. 991-1014
- Related links:
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network. The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it. It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion. This makes the method simple to use but not suitable for simulating digestion kinetics. Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data. This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase. The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix. The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Neurovetenskaper
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Brodkorb, A.
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Egger, L.
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Alminger, Marie, ...
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Alvito, P.
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Assunção, Ricard ...
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Ballance, S.
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show more...
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Bohn, T.
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Bourlieu-Lacanal ...
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Boutrou, R.
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Carriere, F.
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Clemente, Alfons ...
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Corredig, M.
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Dupont, D.
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Dufour, C.
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Edwards, Cathrin ...
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Golding, M.
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Karakaya, S.
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Kirkhus, B.
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Le Feunteun, S.
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Lesmes, U.
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Macierzanka, A.
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Mackie, A.
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Martins, Carla
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Marze, S.
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McClements, D. J ...
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Menard, O.
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Minekus, M.
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Portmann, Reto
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Santos, C. N.
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Souchon, Isabell ...
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Singh, R. Paul
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Vegarud, G. E.
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Wickham, M. S. J ...
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Weitschies, W.
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Recio, I.
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
- Articles in the publication
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Nature Protocols
- By the university
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Chalmers University of Technology