Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene Clusters

Since the discovery of penicillin, natural products and their derivatives have been a valuable resource for drug discovery. With recent development of genome mining approaches in the post-genome era, a great number of natural product biosynthetic gene clusters (BGCs) have been identified and these can potentially be exploited for the discovery of novel natural products that can find application as pharmaceuticals. Since many BGCs are silent or do not express in native hosts under laboratory conditions, heterologous expression of BGCs in genetically tractable hosts becomes an attractive route to activate these BGCs to discover the corresponding products. Here, we highlight recent achievements in cloning and discovery of natural product biosynthetic pathways via intact BGC capturing, and discuss the prospects of high-throughput and multiplexed cloning of rational-designed gene clusters in the future.

Nielsen, Jens B,1962Chalmers tekniska högskola,Chalmers University of Technology,BioInnovation Institute (BII),Danmarks Tekniske Universitet,Technical University of Denmark,Beijing University of Chemical Technology(Swepub:cth)nielsenj (författare)
Liu, Zihe,1984Beijing University of Chemical Technology(Swepub:cth)zihe (författare)
Beijing University of Chemical TechnologyChalmers tekniska högskola (creator_code:org_t)

Ingår i:Frontiers in Bioengineering and Biotechnology: Frontiers Media SA82296-4185

Av författaren/redakt...: Lin, Zhenquan; Nielsen, Jens B, ...; Liu, Zihe, 1984

Om ämnet

TEKNIK OCH TEKNOLOGIER: TEKNIK OCH TEKNO ...; och Maskinteknik; och Produktionstekni ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.