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Reprogramming metha...
Reprogramming methanol utilization pathways to convert Saccharomyces cerevisiae to a synthetic methylotroph
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- Zhan, Chunjun, 1986 (author)
- Joint BioEnergy Institute, California,Chalmers tekniska högskola,Chalmers University of Technology,Jiangnan University,Lawrence Berkeley National Laboratory
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- Li, Xiaowei, 1986 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Lan, Guangxu (author)
- Lawrence Berkeley National Laboratory,Joint BioEnergy Institute, California
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- Baidoo, Edward E.K. (author)
- Lawrence Berkeley National Laboratory,Joint BioEnergy Institute, California
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- Yang, Yankun (author)
- Jiangnan University
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- Liu, Yuzhong (author)
- Joint BioEnergy Institute, California,Lawrence Berkeley National Laboratory
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- Sun, Yang (author)
- Jiangnan University,Henan University of Chinese Medicine
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- Wang, Shijie (author)
- Jiangnan University
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- Wang, Yanyan, 1989 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Wang, Guokun, 1988 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Nielsen, Jens B, 1962 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Keasling, J.D. (author)
- Joint BioEnergy Institute, California,University of California,Lawrence Berkeley National Laboratory,Danmarks Tekniske Universitet,Technical University of Denmark
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- Chen, Yun, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Bai, Zhonghu (author)
- Jiangnan University
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(creator_code:org_t)
- 2023
- 2023
- English.
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In: Nature Catalysis. - 2520-1158. ; 6:5, s. 435-450
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https://doi.org/10.1...
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Abstract
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- Methanol, an organic one-carbon (C1) compound, represents an attractive alternative carbon source for microbial fermentation. Despite considerable advancements in methanol utilization by prokaryotes such as Escherichia coli, engineering eukaryotic model organisms such as Saccharomyces cerevisiae into synthetic methylotrophs remains challenging. Here, an engineered module circuit strategy combined with adaptive laboratory evolution was applied to engineer S. cerevisiae to use methanol as the sole carbon source. We revealed that the evolved glyoxylate-based serine pathway plays an important role in methanol-dependent growth by promoting formaldehyde assimilation. Further, we determined that the isoprenoid biosynthetic pathway was upregulated, resulting in an increased concentration of squalene and ergosterol in our evolved strain. These changes could potentially alleviate cell membrane damage in the presence of methanol. This work sets the stage for expanding the potential of exploiting S. cerevisiae as a potential organic one-carbon platform for biochemical or biofuel production. [Figure not available: see fulltext.].
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik -- Annan kemiteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering -- Other Chemical Engineering (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
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- By the author/editor
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Zhan, Chunjun, 1 ...
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Li, Xiaowei, 198 ...
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Lan, Guangxu
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Baidoo, Edward E ...
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Yang, Yankun
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Liu, Yuzhong
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show more...
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Sun, Yang
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Wang, Shijie
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Wang, Yanyan, 19 ...
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Wang, Guokun, 19 ...
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Nielsen, Jens B, ...
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Keasling, J.D.
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Chen, Yun, 1978
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Bai, Zhonghu
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Chemical Enginee ...
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and Other Chemical E ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
- Articles in the publication
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Nature Catalysis
- By the university
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Chalmers University of Technology