A standardized protocol for the UV induction of Sulfolobus spindle‑shaped virus 1

• The Fuselloviridae prototype member Sulfolobusspindle-shaped virus 1 is a model of UV-inducibleviruses infecting Crenarchaeota. Previous works on SSV1UV induction were bases on empirically determined parametersthat have not yet been standardized. Thus, in manypeer reviewed literature, it is not clear how the fluence andirradiance have been determined. Here, we describe a protocolfor the UV induction of SSV1 replication, which isbased on the combination of the following instrumentallymonitored parameters: (1) the fluence; (2) the irradiance;(3) the exposure time, and (4) the exposure distance. Withthe aim of finding a good balance between the viral replicationinduction and the host cells viability, UV-irradiatedcultures were monitored for their ability to recover in theaftermath of the UV exposure. This UV irradiation procedurehas been set up using the well-characterized Sulfolobussolfataricus P2 strain as model system to study host–virus interaction.

Subject headings

NATURVETENSKAP  -- Biologi -- Mikrobiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Microbiology (hsv//eng)

Keyword

Sulfolobus spindle-shaped virus · UV induction · Irradiation protocol · Fuselloviridae · Viral titre · Plaque assay · Fluence · Irradiance

Publication and Content Type

art (subject category)

ref (subject category)