Sökning: L773:2052 7276
> (2022) >
Catalytic promiscui...
Catalytic promiscuity of O-methyltransferases from Corydalis yanhusuo leading to the structural diversity of benzylisoquinoline alkaloids
-
- Bu, Junling (författare)
- China Academy of Chinese Medical Sciences
-
- Zhang, Xiuhua (författare)
- China Academy of Chinese Medical Sciences
-
- Li, Qishuang (författare)
- China Academy of Chinese Medical Sciences
-
visa fler...
-
- Ma, Ying (författare)
- China Academy of Chinese Medical Sciences
-
- Hu, Zhimin (författare)
- China Academy of Chinese Medical Sciences
-
- Yang, Jian (författare)
- China Academy of Chinese Medical Sciences
-
- Liu, Xiuyu (författare)
- Henan University of Chinese Medicine
-
- Wang, Ruishan (författare)
- China Academy of Chinese Medical Sciences
-
- Jiao, Xiang, 1990 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Chen, Tong (författare)
- China Academy of Chinese Medical Sciences
-
- Lai, Changjiangsheng (författare)
- China Academy of Chinese Medical Sciences
-
- Cui, Guanghong (författare)
- China Academy of Chinese Medical Sciences
-
- Tang, Jinfu (författare)
- China Academy of Chinese Medical Sciences
-
Kong, Yu (författare)
-
Yang, Lei (författare)
-
Lin, Sheng (författare)
-
- Chen, Yun, 1978 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Guo, Juan (författare)
- China Academy of Chinese Medical Sciences
-
- Huang, Luqi (författare)
- China Academy of Chinese Medical Sciences
-
visa färre...
-
(creator_code:org_t)
- 2022-07-06
- 2022
- Engelska.
-
Ingår i: Horticulture Research. - : Oxford University Press (OUP). - 2662-6810 .- 2052-7276. ; 9
- Relaterad länk:
-
https://research.cha... (primary) (free)
-
visa fler...
-
https://doi.org/10.1...
-
https://research.cha...
-
visa färre...
Abstract
Ämnesord
Stäng
- O-methyltransferases play essential roles in producing structural diversity and improving the biological properties of benzylisoquinoline alkaloids (BIAs) in plants. In this study, Corydalis yanhusuo, a plant used in traditional Chinese medicine due to the analgesic effects of its BIA-active compounds, was employed to analyze the catalytic characteristics of O-methyltransferases in the formation of BIA diversity. Seven genes encoding O-methyltransferases were cloned, and functionally characterized using seven potential BIA substrates. Specifically, an O-methyltransferase (CyOMT2) with highly efficient catalytic activity of both 4′- and 6-O-methylations of 1-BIAs was found. CyOMT6 was found to perform two sequential methylations at both 9- and 2-positions of the essential intermediate of tetrahydroprotoberberines, (S)-scoulerine. Two O-methyltransferases (CyOMT5 and CyOMT7) with wide substrate promiscuity were found, with the 2-position of tetrahydroprotoberberines as the preferential catalytic site for CyOMT5 (named scoulerine 2-O-methyltransferase) and the 6-position of 1-BIAs as the preferential site for CyOMT7. In addition, results of integrated phylogenetic molecular docking analysis and site-directed mutation suggested that residues at sites 172, 306, 313, and 314 in CyOMT5 are important for enzyme promiscuity related to O-methylations at the 6- and 7-positions of isoquinoline. Cys at site 253 in CyOMT2 was proved to promote the methylation activity of the 6-position and to expand substrate scopes. This work provides insight into O-methyltransferases in producing BIA diversity in C. yanhusuo and genetic elements for producing BIAs by metabolic engineering and synthetic biology.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Bu, Junling
-
Zhang, Xiuhua
-
Li, Qishuang
-
Ma, Ying
-
Hu, Zhimin
-
Yang, Jian
-
visa fler...
-
Liu, Xiuyu
-
Wang, Ruishan
-
Jiao, Xiang, 199 ...
-
Chen, Tong
-
Lai, Changjiangs ...
-
Cui, Guanghong
-
Tang, Jinfu
-
Kong, Yu
-
Yang, Lei
-
Lin, Sheng
-
Chen, Yun, 1978
-
Guo, Juan
-
Huang, Luqi
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
-
- TEKNIK OCH TEKNOLOGIER
-
TEKNIK OCH TEKNO ...
-
och Industriell biot ...
-
och Biokatalys och e ...
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Kemi
-
och Organisk kemi
- Artiklar i publikationen
-
Horticulture Res ...
- Av lärosätet
-
Chalmers tekniska högskola