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Survey of bromodeox...
Survey of bromodeoxyuridine uptake among environmental bacteria and variation in uptake rates in a taxonomically diverse set of bacterial isolates
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- Hellman, Maria (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology
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- Hallin, Sara (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology
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(creator_code:org_t)
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- Elsevier BV, 2011
- 2011
- English.
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In: Journal of Microbiological Methods. - : Elsevier BV. - 0167-7012 .- 1872-8359. ; 86, s. 376-378
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https://res.slu.se/i...
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Abstract
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- Incorporation of 5-Bromo-2'-Deoxyuridine (BrdU) into DNA can be used to target replicating bacteria in the environment, but differential uptake capacity is a potential bias. Among 23 bacterial isolates commonly found in soils, most took up BrdU, but at up to 10-fold different cell-specific rates. Combined with results from an in silico analysis of 1000 BrdU-labeled 16S rRNA gene sequences, our results demonstrate a BrdU uptake bias with no apparent relationship between taxa affiliation and ability to incorporate BrdU. (C) 2011 Elsevier B.V. All rights reserved.
Subject headings
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Jordbruksvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Agricultural Science (hsv//eng)
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Fisk- och akvakulturforskning (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Fish and Aquacultural Science (hsv//eng)
- LANTBRUKSVETENSKAPER -- Annan lantbruksvetenskap -- Miljö- och naturvårdsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Other Agricultural Sciences -- Environmental Sciences related to Agriculture and Land-use (hsv//eng)
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