Comparison of Sampling Sites and Laboratory Diagnostic Tests for S. equi subsp. equi in Horses from Confirmed Strangles Outbreaks

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Lindahl, SusanneSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences (author)

Comparison of Sampling Sites and Laboratory Diagnostic Tests for S. equi subsp. equi in Horses from Confirmed Strangles Outbreaks

Article/chapterEnglish2013

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2013-03-25
Wiley,2013
Wiley Open Access: Creative Commons Attribution Non-Commercial / Wiley,2024

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LIBRIS-ID:oai:slubar.slu.se:50728
https://res.slu.se/id/publ/50728URI
https://doi.org/10.1111/jvim.12063DOI

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Language:English
Summary in:English

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Subject category:art swepub-publicationtype

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Background Strangles is a contagious equine-specific disease caused by Streptococcus equi subsp. equi. Unfortunately, detection of S. equi can fail in up to 40% of horses with strangles. Whereas recent molecular biologic methods and sampling techniques have improved recovery of S. equi optimal sampling methods and laboratory analyses remain ill-defined. Objectives To determine the yield of S. equi from horses with acute strangles in confirmed outbreaks by field-sampling methods subjected to culture and biochemical identification, and real-time PCR directly and after culture. Animals Fifty-seven horses of varying breeds and ages from 8 strangles outbreaks. Methods Prospective study. Culture with biochemical identification and real-time PCR directly, and from culture, were performed on nasal swabs, nasopharyngeal swabs, and nasopharyngeal lavages. Results Real-time PCR directly from samples identified the highest number of infected horses, with 45/57 nasal swabs, 41/57 nasopharyngeal swabs, and 48/57 nasopharyngeal lavages S. equi positive. Biochemical identification (highest positives 22/57) was inferior to real-time PCR for S. equi recovery regardless of sampling method. Real-time PCR of nasopharyngeal lavage directly and after culture yielded 52/57 positives whereas direct real-time PCR of nasopharyngeal lavage combined with either nasopharyngeal swabs or nasal swabs yielded 53/57 positives. Three horses were negative on all samples. Conclusions and Clinical Importance Nasopharyngeal lavage analyzed by a combination of real-time PCR directly and after culture or, alternatively, real-time PCR directly on a nasopharyngeal lavage and a nasal/nasopharyngeal swab can identify S. equi in over 90% of acute strangles cases.

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Egenvall, AgnetaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences(Swepub:slu)48207 (author)
Båverud, VivecaNational Veterinary Institute (SVA)(Swepub:slu)92814 (author)
Aspán, AnnaNational Veterinary Institute (SVA) (author)
Pringle, JohnSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences(Swepub:slu)48796 (author)
Sveriges lantbruksuniversitetInstitutionen för kliniska vetenskaper (KV) (creator_code:org_t)
Sveriges lantbruksuniversitet

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In:Journal of Veterinary Internal Medicine: Wiley27, s. 542-5470891-66401939-1676

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By the author/editor: Lindahl, Susanne; Egenvall, Agneta; Båverud, Viveca; Aspán, Anna; Pringle, John

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AGRICULTURAL SCIENCES: AGRICULTURAL SCI ...; and Veterinary Scien ...; and Clinical Science

AGRICULTURAL SCIENCES: AGRICULTURAL SCI ...; and Veterinary Scien ...; and Other Veterinary ...

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By the university: Swedish University of Agricultural Sciences

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