Search: WFRF:(Dixelius Christina) >
A novel role of PR2...
A novel role of PR2 in abscisic acid (ABA) mediated, pathogen-induced callose deposition in Arabidopsis thaliana
-
- Bejai, Sarosh (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology
-
- Oide, Shinichi (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology
-
- Guan, Na (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology
-
show more...
-
- Dixelius, Christina (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology
-
show less...
-
(creator_code:org_t)
-
- 2013-08-19
- 2013
- English.
-
In: New Phytologist. - : Wiley. - 0028-646X .- 1469-8137. ; 200, s. 1187-1199
- Related links:
-
https://res.slu.se/i...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Pathogenesis-related protein 2 (PR2) is known to play a major role in plant defense andgeneral stress responses. Resistance against the fungal pathogen Leptosphaeria maculans inArabidopsis requires abscisic acid (ABA), which promotes the deposition of callose, a b-1,3-glucan polymer. Here, we examined the role of PR2 in callose deposition in relation to ABAtreatment and challenge with L. maculans and Pseudomonas syringae. Characterization of PR2-overexpressing plants and the knockout line indicated that PR2negatively affects callose deposition. Recombinant PR2 purified from Pichia pastoris showedcallose-degrading activity, and a considerable reduction in the callose-degrading activity wasobserved in the leaf extract of the PR2 knockout line compared with the wild-type. ABA pretreatment before challenge with L. maculans concomitantly repressed PR2 andenhanced callose accumulation. Likewise, overexpression of an ABA biosynthesis geneNCED3 resulted in reduced PR2 expression and increased callose deposition. We propose that ABA promotes callose deposition through the transcriptional repression ofPR2 in Arabidopsis challenged by L. maculans and P. syringae. Callose by itself is likely to actantagonistically on salicylic acid (SA) defense signaling, suggesting that PR2 may function as amodulator of callose- and SA-dependent defense responses.
Subject headings
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database