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- Aslan, SelcukSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology (author)
Wax esters of different compositions produced via engineering of leaf chloroplast metabolism in Nicotiana benthamiana
- Article/chapterEnglish2014
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- Elsevier BV,2014
- Elsevier,2024
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- LIBRIS-ID:oai:slubar.slu.se:66178
- https://res.slu.se/id/publ/66178URI
- https://doi.org/10.1016/j.ymben.2014.07.001DOI
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- Language:English
- Summary in:English
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- In a future bin based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de nova fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl recluctases (EAR) and wax ester synthases (WS) were transiently expressed in Nic"onana benthamiuna loaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. (C) 2014 The Authors. Published by Elsevier Inc. On behalf of International Metabolic Engineering Society.
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- Sun, ChuanxinSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology(Swepub:slu)47722 (author)
- Leonova, SvetlanaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,VÄXTFÖRÄDLING, Box 101,VÄXTFÖRÄDLING, Box 101(Swepub:slu)50301 (author)
- Dutta, PareshSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för livsmedelsvetenskap,Department of Food Science(Swepub:slu)46840 (author)
- Hofvander, PerSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtförädling,Department of Plant Breeding(Swepub:slu)48735 (author)
- Stymne, StenSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtförädling,Department of Plant Breeding(Swepub:slu)50454 (author)
- Sveriges lantbruksuniversitetInstitutionen för växtbiologi (creator_code:org_t)
- Sveriges lantbruksuniversitet
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- In:Metabolic Engineering: Elsevier BV25, s. 103-1121096-71761096-7184
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