L773:0962 8819
Sökning: L773:0962 8819 > Increased productio...
- Aslan, SelcukSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology (författare)
Increased production of wax esters in transgenic tobacco plants by expression of a fatty acid reductase:wax synthase gene fusion
- Artikel/kapitelEngelska2015
Förlag, utgivningsår, omfång ...
- 2015-07-03
- Springer Science and Business Media LLC,2015
- Springer Verlag (Germany),2024
Nummerbeteckningar
- LIBRIS-ID:oai:slubar.slu.se:69690
- https://res.slu.se/id/publ/69690URI
- https://doi.org/10.1007/s11248-015-9893-5DOI
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- Språk:engelska
- Sammanfattning på:engelska
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- Wax esters are hydrophobic lipids consisting of a fatty acid moiety linked to a fatty alcohol with an ester bond. Plant-derived wax esters are today of particular concern for their potential as cost-effective and sustainable sources of lubricants. However, this aspect is hampered by the fact that the level of wax esters in plants generally is too low to allow commercial exploitation. To investigate whether wax ester biosynthesis can be increased in plants using transgenic approaches, we have here exploited a fusion between two bacterial genes together encoding a single wax ester-forming enzyme, and targeted the resulting protein to chloroplasts in stably transformed tobacco (Nicotiana benthamiana) plants. Compared to wild-type controls, transgenic plants showed both in leaves and stems a significant increase in the total level of wax esters, being eight-fold at the whole plant level. The profiles of fatty acid methyl ester and fatty alcohol in wax esters were related, and C16 and C18 molecules constituted predominant forms. Strong transformants displayed certain developmental aberrations, such as stunted growth and chlorotic leaves and stems. These negative effects were associated with an accumulation of fatty alcohols, suggesting that an adequate balance between formation and esterification of fatty alcohols is crucial for a high wax ester production. The results show that wax ester engineering in transgenic plants is feasible, and suggest that higher yields may become achieved in the near future.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
- Hofvander, PerSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology(Swepub:slu)48735 (författare)
- Dutta, PareshSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för livsmedelsvetenskap,Department of Food Science(Swepub:slu)46840 (författare)
- Sun, ChuanxinSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology(Swepub:slu)47722 (författare)
- Sitbon, FolkeSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology(Swepub:slu)49785 (författare)
- Sveriges lantbruksuniversitetInstitutionen för växtbiologi (creator_code:org_t)
- Sveriges lantbruksuniversitet
Sammanhörande titlar
- Ingår i:Transgenic Research: Springer Science and Business Media LLC24, s. 945-9530962-88191573-9368
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- Aslan, Selcuk
- Hofvander, Per
- Dutta, Paresh
- Sun, Chuanxin
- Sitbon, Folke
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