C-13 Tracking after (CO2)-C-13 Supply Revealed Diurnal Patterns of Wood Formation in Aspen

Mahboubi, MiramirhosseinSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology (author)

Wood of trees is formed from carbon assimilated in the photosynthetic tissues. Determining the temporal dynamics of carbon assimilation, subsequent transport into developing wood, and incorporation to cell walls would further our understanding of wood formation in particular and tree growth in general. To investigate these questions, we designed a (CO2)-C-13 labeling system to study carbon transport and incorporation to developing wood of hybrid aspen (Populus tremula 3 tremuloides). Tracking of C-13 incorporation to wood over a time course using nuclear magnetic resonance spectroscopy revealed diurnal patterns in wood cell wall biosynthesis. The dark period had a differential effect on C-13 incorporation to lignin and cell wall carbohydrates. No C-13 was incorporated into aromatic amino acids of cell wall proteins in the dark, suggesting that cell wall protein biosynthesis ceased during the night. The results show previously unrecognized temporal patterns in wood cell wall biosynthesis, suggest diurnal cycle as a possible cue in the regulation of carbon incorporation to wood, and establish a unique C-13 labeling method for the analysis of wood formation and secondary growth in trees.

Linden, PernillaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology(Swepub:slu)47344 (author)
Moritz, ThomasSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology(Swepub:slu)49634 (author)
Niittylä, TotteSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology(Swepub:slu)49276 (author)
Mahboubi, Amir (author)
Hedenström, MattiasUmeå universitet,Kemiska institutionen(Swepub:umu)mashem96 (author)
Sveriges lantbruksuniversitetInstitutionen för skoglig genetik och växtfysiologi (creator_code:org_t)
Sveriges lantbruksuniversitet

In:Plant Physiology: Oxford University Press (OUP)168, s. 478-4890032-08891532-2548

By the author/editor: Mahboubi, Mirami ...; Linden, Pernilla; Moritz, Thomas; Niittylä, Totte; Mahboubi, Amir; Hedenström, Matt ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Developmental Bi ...

By the university: Swedish University of Agricultural Sciences; Umeå University

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