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- Andreasson, ErikSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Växtskyddsbiologi,Department of Plant Protection Biology (author)
Isolation of Apoplast
- Article/chapterEnglish2017
Publisher, publication year, extent ...
- 2016-10-12
- New York, NY :Springer New York,2017
- Humana Press,2024
Numbers
- LIBRIS-ID:oai:slubar.slu.se:77554
- ISBN:9781493965311
- https://res.slu.se/id/publ/77554URI
- https://doi.org/10.1007/978-1-4939-6533-5_18DOI
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- Language:English
- Summary in:English
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- The apoplast can be described as the soluble fraction of the extracellular space of plant tissue, and it plays an important role in signaling, nutrient transport, and plant-pathogen interactions. In this protocol, we describe a method where leaves are infiltrated with phosphate buffer under vacuum. The apoplast can then be extracted by centrifugation and simultaneously collected in a protease inhibitor solution. Using this protocol, typically 3 mu g of apoplastic proteins can be obtained in a volume of 300 mu L from five potato leaflets, with minimal contamination by non-apoplastic proteins.
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- Abreha, Kibrom BerheSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Växtskyddsbiologi,Department of Plant Protection Biology(Swepub:slu)93268 (author)
- Resjö, SvanteSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Växtskyddsbiologi,Department of Plant Protection Biology(Swepub:slu)46984 (author)
- Sveriges lantbruksuniversitetVäxtskyddsbiologi (creator_code:org_t)
- Sveriges lantbruksuniversitet
Related titles
- In:Methods in Molecular BiologyNew York, NY : Springer New York1511, s. 233-2401064-37451940-6029
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