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Isolation of Apoplast
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Andreasson, ErikSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Växtskyddsbiologi,Department of Plant Protection Biology
(author)
Isolation of Apoplast
- Article/chapterEnglish2017
Publisher, publication year, extent ...
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2016-10-12
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New York, NY :Springer New York,2017
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Humana Press,2024
Numbers
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LIBRIS-ID:oai:slubar.slu.se:77554
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ISBN:9781493965311
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https://res.slu.se/id/publ/77554URI
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https://doi.org/10.1007/978-1-4939-6533-5_18DOI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The apoplast can be described as the soluble fraction of the extracellular space of plant tissue, and it plays an important role in signaling, nutrient transport, and plant-pathogen interactions. In this protocol, we describe a method where leaves are infiltrated with phosphate buffer under vacuum. The apoplast can then be extracted by centrifugation and simultaneously collected in a protease inhibitor solution. Using this protocol, typically 3 mu g of apoplastic proteins can be obtained in a volume of 300 mu L from five potato leaflets, with minimal contamination by non-apoplastic proteins.
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Abreha, Kibrom BerheSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Växtskyddsbiologi,Department of Plant Protection Biology(Swepub:slu)93268
(author)
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Resjö, SvanteSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Växtskyddsbiologi,Department of Plant Protection Biology(Swepub:slu)46984
(author)
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Sveriges lantbruksuniversitetVäxtskyddsbiologi
(creator_code:org_t)
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Sveriges lantbruksuniversitet
Related titles
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In:Methods in Molecular BiologyNew York, NY : Springer New York1511, s. 233-2401064-37451940-6029
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