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Properties of a HeL...
Properties of a HeLa cell 3' exonuclease specific for degrading poly(A) tails of mammalian mRNA.
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- Åström, Jonas (author)
- Uppsala universitet,Medicinsk genetik
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- Åström, Anders (author)
- Uppsala universitet,Medicinsk genetik
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- Virtanen, Anders (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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(creator_code:org_t)
- 1992
- 1992
- English.
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In: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 267:25, s. 18154-18159
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Abstract
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- A HeLa cell 3'-exonuclease with properties of a mammalian mRNA poly(A) tail-removing enzyme has been characterized. The exonuclease shows high specificity for the poly(A) tail, and it is single strand-specific and requires a 3'-hydroxyl group for its activity. During degradation 5'-AMP is liberated as a product, and a 3'-OH group is left on the last adenosine residue of the remaining poly(A) tail. The activity is inhibited by 5'-AMP and can be competed by poly(A)-containing mRNA or poly(A). Based on these findings we propose a reaction pathway for poly(A) tail removal catalyzed by the HeLa cell poly(A) tail-specific 3' exonuclease.
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