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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 05181naa a2200397 4500 | |
| 001 | oai:DiVA.org:kth-297831 | |
| 003 | SwePub | |
| 008 | 210629s2008 | |||||||||||000 ||kor| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2978312 URI |
| 040 | a (SwePub)kth | |
| 041 | a korb engb kor | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Jo, Ji Songu Department of Chemical & Biological Engineering and ERI, GyeongSang National University4 aut |
| 245 | 1 0 | a Anti-wrinkle activity of beta-carotene extracted & purified from recombinant Escherichia coli |
| 264 | 1 | c 2008 |
| 338 | a print2 rdacarrier | |
| 500 | a QC 20210816 | |
| 520 | a This paper described the extraction/purification of β-carotene from recombinant E.coli and evaluation of anti-wrinkle activity of purified β-carotene. No significant differences in extraction yields were observed when hexane or isobutyl acetate was used. However, extraction from wet-cell cake resulted in 2-fold higher amount of β-carotene than that from dry cells. Disruption of 5 g-wet cells by ultrasonic homogenizer, acetone dehydration, extraction with isobutyl acetate resulted in 36 mg of β-carotene corresponding to 61.2% of recovery. The formation and separation of ββ-carotene crystal improved the purity. 633 mg of β-carotene crystal with 93% purity was obtained from 223 g/L of wet-cell cake harvested from 2.5-L fed-batch culture broth. The cultures of normal human primary fibroblast were performed to investigate the effect of β-carotene on cytotoxicity as MTT assay and anti-wrinkle activity as collagen synthesis assays. 1.7μM of β-carotene was found to be optimal concentration at which 1.4-fold higher amount of collagen was synthesized than that in absence of β-carotene. This indicates that highly purified β-carotene can be obtained from recombinant E.coli by applying simple method with less toxic solvent and can be used in functional cosmetics as anti-wrinkle agent. | |
| 520 | a 재조합 대장균으로부터 베타-카로틴을 추출/정제하고 정제된 베타-카로틴의 주름개선 성능을 평가하였다. 탈수 용매, 추출 용매에 따른 추출량의 큰 변화는 발견되지 않았으나, wet-cell cake으로부터 추출한 경우가 dry cell로부터 추출한 경우에 비하여 2배 많은 양의 베타-카로틴이 추출되었다. 5 g의 wet-cell cake을 파쇄하고, 아세톤으로 탈수한 후, isobutyl acetate로 추출한 결과 36 mg의 베타-카로틴이 추출되었는데, 이는 61.2% 회수율에 해당한다. 베타-카로틴의 결정을 생성시키고 이를 분리한 결과 순도가 증가하였다. 재조합 대장균 배양액 2.5 L로부터 얻은 wet-cell cake 223 g의 탈수, 세포 파쇄 후 세포 잔사물로부터 베타-카로틴 추출, 농축, 결정화 등을 통하여 최종적으로 순도 93%인 베타-카로틴 결정 633 mg을 얻었다. 사람섬유아세포 배양을 통하여 정제된 베타-카로틴의 세포 독성 및 콜라겐 합성에 미치는 영향을 조사하였다. 1.7μM1.7μM의 베타-카로틴을 첨가 시 세포에 독성을 거의 나타내지 않았으며 콜라겐 합성양은 첨가하지 않은 경우에 비하여 1.4배 증가하였다. 본 결과는 저 유독성 용매를 사용하고 기존보다 간단한 방법을 사용하여 재조합 대장균으로부터 고순도의 베타-카로틴을 정제할 수 있고 이를 기능성 화장품의 주름개선제로 사용될 수 있음을 시사한다. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 700 | 1 | a Ku, Bo Miu Department of Anatomy & Neurobiology, GyeongSang National University4 aut |
| 700 | 1 | a Kang, Sang Soou Department of Anatomy & Neurobiology, GyeongSang National University4 aut |
| 700 | 1 | a Lee, Jae Ranu Department of Chemical & Biological Engineering and ERI, GyeongSang National University4 aut |
| 700 | 1 | a Kim, You Geunu KB Cosmetics4 aut |
| 700 | 1 | a Li, He,d 1985-u Department of Chemical & Biological Engineering and ERI, GyeongSang National University4 aut0 (Swepub:kth)u192ayff |
| 700 | 1 | a Kim, Sung-Baeu (Department of Chemical & Biological Engineering and ERI, GyeongSang National University4 aut |
| 700 | 1 | a Kim, Seon-Wonu Division of Applied Life Science, GyeongSang National University4 aut |
| 700 | 1 | a Kim, Chang-Joonu Department of Chemical & Biological Engineering and ERI, GyeongSang National University4 aut |
| 700 | 1 | a Chung, In-Youngu Department of Electronics and Communications, Kwangwoon University4 aut |
| 710 | 2 | a Department of Chemical & Biological Engineering and ERI, GyeongSang National Universityb Department of Anatomy & Neurobiology, GyeongSang National University4 org |
| 773 | 0 | t KSBB Journalg 23:6, s. 513-518q 23:6<513-518x 1225-7117x 2288-8268 |
| 856 | 4 | u https://www.koreascience.or.kr/article/JAKO200811237152656.page |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-297831 |
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