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Probing physical pr...
Probing physical properties of DNA-protein complexes using nanofluidic channels
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- Frykholm, Karolin, 1977 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Alizadehheidari, Mohammadreza, 1987 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Fornander, Louise, 1984 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Fritzsche, Joachim, 1977 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Wigenius, Jens, 1975 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Beuning, P. J. (author)
- Northeastern University
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- Modesti, M. (author)
- Aix-Marseille Université,Aix Marseille University
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- Persson, F. (author)
- Uppsala universitet,Uppsala University
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- Westerlund, Fredrik, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2013
- 2013
- English.
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In: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013. - 9781632666246 ; 2, s. 1311-1313
- Related links:
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https://research.cha...
Abstract
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- We present the use of nanofluidic channels as a tool for determining physical properties of single DNA-protein complexes. By coating the nanochannels with a lipid bilayer we avoid sticking of proteins to the channel walls. RecA is a prokaryotic protein involved in recombination and DNA repair. We study filaments of RecA, bound to both double stranded (ds) and single stranded (ss) DNA. We determine the persistence length of RecA filaments on both dsDNA and ssDNA and obtain values in agreement with the literature. Neither the DNA nor the protein has to be attached to handles or surfaces, and the technique is directly transferable to Lab-on-a-Chip technologies for high throughput measurements in solution.
Subject headings
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
Keyword
- DNA-protein interactions
- Single DNA molecules
- Lab-on-a-Chip
- Nanofluidics
Publication and Content Type
- kon (subject category)
- ref (subject category)
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Frykholm, Karoli ...
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Alizadehheidari, ...
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Fornander, Louis ...
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Fritzsche, Joach ...
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Wigenius, Jens, ...
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Beuning, P. J.
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Modesti, M.
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Persson, F.
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Westerlund, Fred ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Physical Chemist ...
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Nano technology
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17th Internation ...
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Chalmers University of Technology