SwePub
Sök i LIBRIS databas

  Utökad sökning

(WFRF:(Gonchar M.))
 

Sökning: (WFRF:(Gonchar M.)) > (2010-2014) > Formaldehyde-sensit...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003878naa a2200433 4500
001oai:lup.lub.lu.se:edf7adae-277b-4179-8db2-221b7ef4d5c5
003SwePub
008160401s2010 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/16723272 URI
0247 a https://doi.org/10.1007/s00604-010-0327-z2 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Korpan, Yaroslav I.4 aut
2451 0a Formaldehyde-sensitive conductometric sensors based on commercial and recombinant formaldehyde dehydrogenase
264 c 2010-03-21
264 1b Springer Science and Business Media LLC,c 2010
520 a Novel formaldehyde-sensitive conductometric biosensors have been developed that are based on commercial bacterial formaldehyde dehydrogenase (FDH) from Pseudomonas putida and recombinant formaldehyde dehydrogenase (rFDH) from the yeast Hansenula polymorpha as the bio-recognition elements. The bio-recognition membranes have mono-layer architecture and consist of enzyme cross-linked with albumin and of the cofactors NAD (for FDH-based sensor) or NAD and glutathione (for rFDH-based sensor). This architecture of the biosensor allows the determination of formaldehyde without adding NAD and glutathione to the analyzed sample at every analysis and conducting measurements on the same transducer without cofactors regeneration since the bio-membrane contains it at high concentration (100 mM for NAD and 20 mM for glutathione). The response is linear in the range from 10 to 200 mM of formaldehyde concentration depending on the enzyme used. The dependence of the biosensor output signals on pH and buffer concentration as well as operational/storage stability and selectivity/specificity of the developed conductometric biosensors have been investigated. The relative standard deviation of the intra-sensor response did not exceed 4% and 10% for rFDH- and FDH-based sensors, respectively. The relative standard deviation of the inter-sensor response constituted 20% for both dehydrogenases used. The biosensors have been validated for formaldehyde detection in some real samples of pharmaceutical (Formidron), disinfectant (Descoton forte) and an industrial product (Formalin). A good correlation does exist between the concentration values measured by the conductometric biosensor developed in this work, an enzymatic method, amperometric biosensors developed earlier, and standard analytical methods of formaldehyde determination.
650 7a NATURVETENSKAPx Kemix Analytisk kemi0 (SwePub)104012 hsv//swe
650 7a NATURAL SCIENCESx Chemical Sciencesx Analytical Chemistry0 (SwePub)104012 hsv//eng
653 a Formaldehyde dehydrogenase
653 a biosensor
653 a Thin-film interdigitated gold planar electrodes
653 a Conductometric
653 a Formaldehyde detection
7001 a Soldatkin, Olexandr O.4 aut
7001 a Sosovska, Olga F.4 aut
7001 a Klepach, Halyna M.4 aut
7001 a Csöregi, Elisabethu Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)akem-ecs
7001 a Vocanson, Francis4 aut
7001 a Jaffrezic-Renault, Nicole4 aut
7001 a Gonchar, Mykhailo V.4 aut
7102 a Centrum för analys och syntesb Kemiska institutionen4 org
7730 t Microchimica Actad : Springer Science and Business Media LLCg 170:3-4, s. 337-344q 170:3-4<337-344x 1436-5073x 0026-3672
8564 u http://dx.doi.org/10.1007/s00604-010-0327-zy FULLTEXT
8564 8u https://lup.lub.lu.se/record/1672327
8564 8u https://doi.org/10.1007/s00604-010-0327-z

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy