(WFRF:(Loman Niklas))
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G proteins coupled to phospholipase C: molecular targets of long-term ethanol exposure
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- Simonsson, Per (author)
- Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups
- Rodriguez, F David (author)
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- Loman, Niklas (author)
- Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- (creator_code:org_t)
- Wiley, 1991
- 1991
- English.
- In: Journal of Neurochemistry. - : Wiley. - 1471-4159 .- 0022-3042. ; 56:6, s. 2018-2026
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Long-term ethanol exposure is known to inhibit bradykinin-stimulated phosphoinositide hydrolysis in cultures of neuroblastoma x glioma 108-15 cells. In the present study, [3H]bradykinin binding, GTP-binding protein function, and phospholipase C activity were assayed in cells grown for 4 days in 100 mM ethanol with the aim of elucidating the molecular target of ethanol on signal transduction coupled to inositol trisphosphate and diacylglycerol formation. Ethanol exposure reduced guanosine 5'-O-(3-thiotriphosphate) [GTP(S)]- and, to a lesser extent, NaF/AlCl3-stimulated phosphoinositide hydrolysis, whereas it had no effect on the enzymatic activity of a phosphatidylinositol 4,5-bisphosphate-specific phospholipase C. [3H]Bradykinin binding in the absence of GTP(S) was not influenced by ethanol exposure. However, the reduction in [3H]bradykinin binding seen in control cells after addition of GTP analogue was inhibited in cells grown in ethanol-containing medium. The results indicate that long-term ethanol exposure exerts its effects on receptor-stimulated phosphoinositide hydrolysis primarily at the level of the GTP-binding protein.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Bradykinin
- Ethanol
- Phosphatidylinositol 4
- 5-bisphosphate
- Phospholipase C
- GTP-binding protein
- NG 108–15 cell line
Publication and Content Type
- art (subject category)
- ref (subject category)
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- Loman, Niklas
- Alling, Christer
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- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
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