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Cofilin regulator 1...
Cofilin regulator 14-3-3zeta is an evolutionarily conserved protein required for phagocytosis and microbial resistance
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Ulvila, Johanna (author)
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Vanha-aho, Leena-Maija (author)
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- Kleino, Anni (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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Vähä-Mäkilä, Mari (author)
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Vuoksio, Milka (author)
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Eskelinen, Sinikka (author)
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- Hultmark, Dan (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Hultmark
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Kocks, Christine (author)
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Hallman, Mikko (author)
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Parikka, Mataleena (author)
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Rämet, Mika (author)
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(creator_code:org_t)
- 2011-01-04
- 2011
- English.
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In: Journal of Leukocyte Biology. - : Society for Leukocyte Biology. - 0741-5400 .- 1938-3673. ; 89:5, s. 649-659
- Related links:
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https://jlb.onlineli...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Phagocytosis is an ancient cellular process that plays an important role in host defense. In Drosophila melanogaster phagocytic, macrophage-like hemocytes recognize and ingest microbes. We performed an RNAi-based in vitro screen in the Drosophila hemocyte cell line S2 and identified Abi, cpa, cofilin regulator 14-3-3ζ, tlk, CG2765, and CG15609 as mediators of bacterial phagocytosis. Of these identified genes, 14-3-3ζ had an evolutionarily conserved role in phagocytosis: bacterial phagocytosis was compromised when 14-3-3ζ was targeted with RNAi in primary Drosophila hemocytes and when the orthologous genes Ywhab and Ywhaz were silenced in zebrafish and mouse RAW 264.7 cells, respectively. In Drosophila and zebrafish infection models, 14-3-3ζ was required for resistance against Staphylococcus aureus. We conclude that 14-3-3ζ is essential for phagocytosis and microbial resistance in insects and vertebrates.
Keyword
- Drosophila
- zebrafish
- plasmatocyte
- macrophage
- immunity
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Ulvila, Johanna
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Vanha-aho, Leena ...
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Kleino, Anni
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Vähä-Mäkilä, Mar ...
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Vuoksio, Milka
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Eskelinen, Sinik ...
-
show more...
-
Hultmark, Dan
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Kocks, Christine
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Hallman, Mikko
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Parikka, Matalee ...
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Rämet, Mika
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show less...
- Articles in the publication
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Journal of Leuko ...
- By the university
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Umeå University