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“Molecular Activity...
“Molecular Activity Painting” : Switch-like, light-controlled perturbations inside living cells
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Chen, Xi (author)
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Venkatachalapathy, Muthukumaran (author)
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Kamps, Dominic (author)
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Weigel, Simone (author)
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Kumar, Ravi (author)
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Orlich, Michael (author)
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Garrecht, Ruben (author)
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Hirtz, Michael (author)
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Niemeyer, Christof M. (author)
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- Wu, Yao-Wen, Professor (author)
- Chemical Genomics Centre of the Max-Planck Society, Dortmund, Germany
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Dehmelt, Leif (author)
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(creator_code:org_t)
- 2017-03-29
- 2017
- English.
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In: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 56:21, s. 5916-5920
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http://umu.diva-port...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Acute subcellular protein targeting is a powerful tool to study biological networks. However, signaling at the plasma membrane is highly dynamic, making it difficult to study in space and time. In particular, sustained local control of molecular function is challenging due to lateral diffusion of plasma membrane targeted molecules. Here we present “Molecular Activity Painting” (MAP), a novel technology which combines photoactivatable chemically induced dimerization (pCID) with immobilized artificial receptors. The immobilization of artificial receptors by surface-immobilized antibodies blocks lateral diffusion, enabling rapid and stable “painting” of signaling molecules and their activity at the plasma membrane with micrometer precision. Using this method, we show that painting of the RhoA-myosin activator GEF-H1 induces patterned acto-myosin contraction inside living cells.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Keyword
- Optochemical biology
- Photochemically induced dimerization
- Rho GTPases
- Signal transduction
- Immobilization
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Chen, Xi
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Venkatachalapath ...
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Kamps, Dominic
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Weigel, Simone
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Kumar, Ravi
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Orlich, Michael
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show more...
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Garrecht, Ruben
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Hirtz, Michael
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Niemeyer, Christ ...
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Wu, Yao-Wen, Pro ...
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Dehmelt, Leif
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Organic Chemistr ...
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Angewandte Chemi ...
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Umeå University